An improved spectrofluorometric determination of selenium in biological materials after microwave digestion

In the present work, a simple spectrofluorometric method for the determination of selenium in biological materials using microwave-assisted digestion was improved. The conditions of reduction of Se(VI) to Se(IV) and its complexation with DAN were studied as well as the excitation and emission wavelengths that provide higher fluorescence intensity. One of the goals in our research work was that no further adjustment of pH was required before solvent extraction since minor fluorescence background in obtained with the intrinsic H+ concentration