Development of analytical methodology for verification of authenticity of apple juice

Graduation date: 1985Apples growing in New Zealand, Argentina, Mexico, Michigan and\ud Washington were used to provide the compositional data and to\ud develop the analytical procedures for the use in verification of\ud authenticity of apple juices.\ud High Performance Liquid Chromatography (HPLC) techniques were\ud applied for glucose, fructose, sucrose, sorbitol, malic, citric,\ud quinic, shikimic, fumaric, chlorogenic acid, and\ud hydroxymethylfurfural (HMF). L-malic acid was determined by an\ud enzymic method. Sep-Pak C18 cartridge, anion exchange resin and\ud millipore filter were used for sample preparation of HPLC.\ud Influence of postharvest storage on sucrose and sorbitol\ud content was observed but it had no effect on the glucose/fructose\ud ratios. Quinic was the next most abundant acid in all the apple\ud varieties examined. Paired t-test showed good agreement (p