Suillus collinitus (Fr.) Kuntze ekstraktlarının insan lenfositlerinin genotoksisitesi ve proliferasyonu üzerindeki etkileri

Cultivated or wild edible mushroom species have traditionally been used by humans for medical purposes for manyyears. Edible mushrooms have the potential to show different activities due to the numerous bioactive components they contain.In particular, some mushroom species whose regulatory properties have been identified on human immunity are of interest in thescientific world. Considering these characteristics of edible mushroom species, in the present study, it was examined the effectsof Suillus collinitus (Fr.) Kuntze, an important edible mushroom species, on human peripheral lymphocytes. For this purpose,acetone and water extracts were obtained from S. collinitus and the effects of these extracts on genotoxicity and proliferation ofhuman lymphocytes were tested by chromosome aberration (CA), micronucleus (MN), nuclear division index (NBI) and mitoticindex (MI) analyses. When genotoxicity analyses were examined, it was found that none of the tested extract applications (1-100 mg/L) did not change the CA and MN frequencies statistically (p > 0.05) compared to the negative control group.Proliferation analyses showed that only the maximum concentration (100 mg/L) application of acetone extract of S. collinitusdecreased the NBI and MI ratio of the cells at a level of p 0.05) olarak değiştirmediği tespit edilmiştir. Proliferasyon analizleri ise yalnızca S. collinitus’un aseton ekstraktının maksimum konsantrasyonlu (100 mg/L) uygulamasının, hücrelerin NBİ ve Mİ oranını negatif kontrol grubuna kıyasla p < 0.05 düzeyinde düşürdüğünü göstermiştir. Elde edilen sonuçlar, S. collinitus’un aseton ve su ekstraktlarının özellikle 1-50 mg/L konsantrasyonlu uygulamalarının, insan bağışıklık sisteminde görevli lenfositler üzerinde herhangi bir genotoksik veya sitotoksik aktivite göstermediğini ortaya çıkarmıştır