Effects of tetrandrine on hepatocarcinoma cell lines.

Yu, Wai Lam.Thesis (M.Phil.)--Chinese University of Hong Kong, 2011.Includes bibliographical references (leaves 79-88).Abstracts in English and Chinese.Acknowledgements --- p.IVAbstract --- p.V論文摘要 --- p.VIITable of Contents --- p.IXChapter Chapter 1 --- Introduction --- p.1Chapter 1.1 --- Cancer --- p.1Chapter 1.2 --- Hepatocellular Carcinoma (HCC) --- p.2Chapter 1.2.1 --- Risk factors causing HCC --- p.3Chapter 1.2.2 --- Molecular mechanism of HCC --- p.7Chapter 1.2.3 --- Treatment of HCC --- p.8Chapter 1.3 --- Tetrandrine (Tet) - A Natural Compound Derived from Traditional Chinese Medicine (TCM) --- p.10Chapter 1.3.1 --- Traditional Chinese Medicine (TCM) --- p.10Chapter 1.3.2 --- Tetrandrine (Tet) --- p.12Chapter 1.4 --- Molecular View of Apoptosis --- p.14Chapter 1.4.1 --- Overview of apoptosis --- p.14Chapter 1.4.2 --- Caspase cascade --- p.15Chapter 1.4.3 --- Bcl-2 protein family --- p.18Chapter 1.4.4 --- The role of mitochondria in apoptosis --- p.20Chapter 1.5 --- Anti-cancer Agents Inducing Apoptosis Are New Targets --- p.22Chapter 1.6 --- Aim of Study --- p.26Chapter Chapter 2 --- Materials and Methods --- p.27Chapter 2.1 --- Cell Culture And Treatment --- p.27Chapter 2.1.1 --- Cell lines used --- p.27Chapter 2.1.2 --- Tetrandrine (Tet) --- p.28Chapter 2.1.3 --- Chemicals and reagents 2 --- p.83Chapter 2.1.4 --- Solution preparation --- p.29Chapter 2.1.5 --- Procedures --- p.30Chapter 2.2 --- Cell viability --- p.32Chapter 2.2.1 --- Chemicals and reagents . --- p.32Chapter 2.2.2 --- Solution preparation --- p.32Chapter 2.2.3 --- Procedures --- p.32Chapter 2.3 --- Apoptosis detection --- p.34Chapter 2.3.1 --- Chemicals and reagents --- p.34Chapter 2.3.2 --- Solution preparation --- p.35Chapter 2.3.3 --- Procedures --- p.36Chapter 2.4 --- Gene expression in tet-induced apoptotic cells --- p.39Chapter 2.4.1 --- Chemicals and reagents --- p.39Chapter 2.4.2 --- Solution preparation --- p.40Chapter 2.4.3 --- Procedures --- p.40Chapter 2.5 --- Protein expression in tet-induced apoptotic cells --- p.44Chapter 2.5.1 --- Chemicals and reagents --- p.44Chapter 2.5.2 --- Solution preparation --- p.45Chapter 2.5.3 --- Procedures --- p.48Chapter 2.6 --- Cell cycle analysis of tet-treated cells --- p.54Chapter 2.5.1 --- Chemicals and reagents --- p.54Chapter 2.5.2 --- Solution preparation --- p.54Chapter 2.5.3 --- Procedures --- p.54Chapter Chapter 3 --- Result --- p.56Chapter Chapter 4 --- Discussion --- p.70Chapter 4.1 --- Dose- and Time- Dependent Inhibitory Effects of Tet were found on HuH-7 And JHH-4 Cell Lines --- p.70Chapter 4.2 --- Tet Is More Selective Towards Liver Cancer Cells --- p.71Chapter 4.3 --- The Cell Death in HuH-7 Cells Induced by Tet is Mediated Through Apoptosis --- p.72Chapter 4.4 --- Hepatocellular Carcinoma (HCC)Tet Induces G1 Phase Cell Cycle Arrest as Part of Its Mechanism in Inducing Apoptosis in HuH-7 Cells --- p.73Chapter 4.5 --- Tet Could Probably Induce G1 Phase Cell Cycle Arrest in JHH-4 Cells --- p.75Chapter 4.6 --- "Tet-induced Apoptosis Involves the Intrinsic, Caspase-Dependent Pathway in Both the HuH-7 and JHH-4 Cell Lines" --- p.75Chapter 4.7 --- Proteins in Bcl-2 Family are Involved in the Inhibitory Mechanism of Tet --- p.77Reference --- p.7