An immunohistologic study of biological parameters in prostatic intraepithelial neoplasia and adenocarcinoma.

by Kwan Yiu Wing.Thesis (M.Phil.)--Chinese University of Hong Kong, 1999.Includes bibliographical references (leaves 167-187).Abstracts in English and Chinese.ACKNOWLEDGMENTS --- p.IVABSTRACT --- p.1Chapter CHAPTER 1. --- INTRODUCTION --- p.5Chapter I. --- Epidemiology of Prostate Cancer --- p.5Chapter II. --- The Normal Prostate - Prostatic Anatomy --- p.9Chapter III. --- Pathology of Prostatic Cancers --- p.12Chapter CHAPTER 2. --- PROSTATIC INTRAEPITHELIAL NEOPLASIA --- p.16Chapter I. --- Introduction --- p.16Chapter II. --- "Definition, Characteristics and Grading" --- p.16Chapter III. --- Incidence and Prevalence of PIN --- p.26Chapter IV. --- Evidence Linking PIN with Prostatic Carcinoma --- p.27Chapter V. --- Conclusion --- p.37Chapter CHAPTER 3. --- HISTOLOGIC BIOMARKERS --- p.39Chapter I. --- p53 Protein --- p.39Chapter II. --- Proliferating Cell Nuclear Antigen (PCNA) --- p.44Chapter III. --- Ki-67 Antigen --- p.48Chapter IV. --- Epidermal Growth Factor Receptor --- p.49Chapter V. --- E-Cadherin --- p.51Chapter VI. --- CD44 --- p.54Chapter VII. --- nm23 --- p.58Chapter CHAPTER 4. --- OBJECTIVES OF STUDY --- p.62Chapter CHAPTER 5. --- MATERIALS AND METHODS --- p.63Chapter I. --- Materials --- p.63Chapter II. --- Methods --- p.71Chapter III. --- Interpretation of Immunostaining Results --- p.78Chapter IV. --- Statistical Analysis --- p.82Chapter CHAPTER 6. --- RESULTS --- p.83Chapter I. --- Immunohistochemical Results for p53 Protein --- p.83Chapter II. --- Results of Immunostaining of PCNA --- p.90Chapter III. --- Immunostaining and Quantitation of Ki-67 Expression --- p.97Chapter IV. --- Immunohistochemical Expression of EGFr --- p.105Chapter V. --- E-Cadherin --- p.110Chapter VI. --- CD44 --- p.115Chapter VII. --- Expression of nm23 in Prostatic Lesions --- p.122Chapter VIII. --- Correlation and Association of Expressions of All Biomarkers in Prostatic Lesions --- p.128Chapter CHAPTER 7. --- DISCUSSION --- p.132Chapter I. --- p53 Protein --- p.135Chapter II. --- PCNA --- p.137Chapter III. --- Ki-67 --- p.140Chapter IV. --- EGFr --- p.143Chapter V. --- E-Cadherin --- p.146Chapter VI. --- CD44 --- p.148Chapter VII. --- nm23 --- p.151Chapter VIII. --- Association between Biomarkers and Prostate lesions --- p.154Chapter CHAPTER 8. --- SUMMARY AND CONCLUSION --- p.157APPENDICES --- p.164Chapter I. --- Table of incidence and mortality rates of prostate cancer in the United States from 1973 to 1995 by race --- p.164Chapter II. --- Table of leading cancer deaths in Hong Kong from 1971 to 1996 --- p.165Chapter III. --- Table of incidence and mortality rate caused by prostate cancer in Hong Kong --- p.166Chapter IV. --- Reagents --- p.167REFERENCES --- p.16