Degeneration of articular cartilage and cartilage healing in rabbit models.

by Linda, Fu Lap Kun.Thesis (M.Phil.)--Chinese University of Hong Kong, 1993.Includes bibliographical references (leaves 115-128).ACKNOWLEDGMENT --- p.iTABLE OF CONTENTS --- p.iiABSTRACT --- p.xiiChapter CHAPTER ONE: --- INTRODUCTION --- p.1Chapter CHAPTER TWO : --- LITERATURE REVIEW --- p.6Chapter 2.1 --- BIOLOGY OF ARTICULAR CARTILAGE --- p.7Chapter 2.1.1 --- Normal cartilage --- p.7Chapter 2.1.1.1 --- Composition and properties --- p.7Chapter 2.1.1.2 --- Morphology of normal cartilage --- p.12Chapter 2.1.2 --- Degeneration of cartilage --- p.12Chapter 2.1.2.1 --- Morphology of degenerative cartilage --- p.12Chapter 2.1.2.2 --- Biochemical characteristics of degenerative cartilage --- p.13Chapter 2.1.3 --- Healing of degenerated articular cartilage --- p.14Chapter 2.2 --- METHODS TO CREATE OSTEOARTHRITIS MODEL --- p.15Chapter 2.2.1 --- Immobilization --- p.16Chapter 2.2.2 --- Instability method --- p.16Chapter 2.3 --- SCORING OF OSTEOARTHRITIS --- p.17Chapter 2.3.1 --- Gross morphology --- p.17Chapter 2.3.1.1 --- Mapping of OA area --- p.17Chapter 2.3.1.2 --- Amount of synovial fluid --- p.18Chapter 2.3.1.3 --- Mobility --- p.18Chapter 2.3.2 --- Histological scoring --- p.18Chapter 2.3.3 --- Biochemical comparison --- p.21Chapter 2.3.3.1 --- Water content --- p.21Chapter 2.3.3.2 --- Proteoglycan --- p.21Chapter 2.4 --- THE TREATMENT OF OSTEOARTHRITIS --- p.23Chapter 2.4.1 --- Biology of Hyaluronic acid --- p.24Chapter 2.4.2 --- Repairing properties of Hyaluronic acid in OA --- p.24Chapter 2.4.3 --- The pharmacology of Hyalgan --- p.26Chapter 2.4.4 --- Intraarticular injection of Hyaluronic acid in animals --- p.26Chapter CHAPTER THREE: --- CREATING OSTEOARTHRITIC MODEL --- p.28Chapter 3.1 --- INTRODUCTION --- p.29Chapter 3.2 --- MATERIAL AND METHODOLOGY --- p.30Chapter 3.2.1 --- Animals --- p.30Chapter 3.2.2 --- Instabilization --- p.32Chapter 3.2.2.1 --- Reagent and Apparatus --- p.32Chapter 3.2.2.2 --- Procedure --- p.32Chapter 3.2.3 --- Immobilization --- p.36Chapter 3.2.3.1 --- Reagent and Apparatus --- p.36Chapter 3.2.3.2 --- Procedure --- p.37Chapter 3.3 --- METHODS OF ASSESSMENT --- p.39Chapter 3.3.1 --- Gross structure comparison --- p.40Chapter 3.3.1.1 --- Gross mapping of the OA areas --- p.40Chapter 3.3.2 --- Histochemical scoring --- p.40Chapter 3.3.2.1 --- Procedure --- p.40Chapter 3.3.2.1.1 --- Hematoxylin & eosin stain --- p.45Chapter 3.3.2.1.2 --- Safranin O stain --- p.45Chapter 3.3.3 --- Biochemical assessment --- p.47Chapter 3.3.3.1 --- Water content --- p.47Chapter 3.3.3.2 --- Proteoglycan content --- p.49Chapter 3.3.3.2.1 --- Reagent --- p.49Chapter 3.3.3.2.2 --- Procedure --- p.49Chapter 3.3.3.2.3 --- Standard curve --- p.52Chapter 3.4 --- METHODS OF STATISTICAL ANALYSIS --- p.53Chapter 3.5 --- RESULTS --- p.54Chapter 3.5.1 --- Gross morphology --- p.54Chapter 3.5.1.1 --- General appearance --- p.54Chapter 3.5.1.2 --- Gross osteoarthritic area --- p.56Chapter 3.5.2 --- Histological results --- p.59Chapter 3.5.2.1 --- Histological appearance --- p.59Chapter 3.5.3 --- Results of biochemical assay --- p.66Chapter 3.3.3.1 --- Water content --- p.66Chapter 3.5.3.2 --- Proteoglycan --- p.69Chapter 3.6 --- DISCUSSION --- p.72Chapter 3.6.1 --- Relation between gross OA area & Mankin's score --- p.72Chapter 3.6.2 --- Relation between gross OA area & GAG content --- p.74Chapter 3.6.3 --- Relation between Mankin's score & water content --- p.76Chapter 3.6.4 --- Relation between Mankin's score & GAG content --- p.78Chapter 3.6.5 --- Comparison of the four different parameters --- p.79Chapter 3.6.6 --- Merit on the four parameters --- p.84Chapter 3.6.6 --- Consistency & reproducible of the OA model --- p.85Chapter 3.6.7 --- Moderately severe OA model was needed in Part II --- p.86Chapter 3.7 --- CONCLUSION --- p.86Chapter CHAPTER FOUR: --- HEALING OF CARTILAGE --- p.87Chapter 4.1 --- INTRODUCTION --- p.88Chapter 4.2 --- MATERIALS AND METHODS --- p.89Chapter 4.2.1 --- Establishing the osteoarthritis --- p.89Chapter 4.2.2 --- Treatment of the rabbits --- p.90Chapter 4.2.2.1 --- Reagent and Apparatus --- p.90Chapter 4.2.2.2 --- Procedure --- p.91Chapter 4.2.3 --- Methods of assessment --- p.92Chapter 4.2.4 --- Methods of statistical analysis --- p.92Chapter 4.3 --- RESULTS --- p.93Chapter 4.3.1 --- Gross OA area --- p.94Chapter 4.3.2 --- Mankin's score --- p.97Chapter 4.3.3 --- Water content --- p.99Chapter 4.3.4 --- Proteoglycan . . --- p.101Chapter 4.3.5 --- Results of Part I and Part II --- p.103Chapter 4.4 --- DISCUSSION --- p.107Chapter 4.4.1 --- Morphological comparison --- p.107Chapter 4.4.2 --- Biochemical comparison --- p.110Chapter 4.4.3 --- Comparison of the results in two parts of experiment --- p.111Chapter 4.5 --- CONCLUSION --- p.113BIBLIOGRAPHY --- p.114APPENDIX --- p.12