Effects of hepato-protective herbal medicines on gene expression in rat hepatocytes and hepatoma cells.

Chan Chun-pong.Thesis (M.Phil.)--Chinese University of Hong Kong, 2002.Includes bibliographical references (leaves 171-176).Abstracts in English and Chinese.Acknowledgements --- p.iAbstract --- p.ii摘要 --- p.iiiAbbreviation --- p.ivTable of contents --- p.vList of figures --- p.xiList of tables --- p.xviChapter Chapter 1 --- introductionChapter 1.1 --- Traditional Chinese medicines (TCMs) --- p.2Chapter 1.2 --- Liver disorders in Asia Pacific region --- p.3Chapter 1.3 --- Classification of liver disorders --- p.7Chapter 1.3.1 --- Hepatitis --- p.8Chapter 1.3.1.1 --- Hepatitis A virus infection --- p.8Chapter 1.3.1.2 --- Hepatitis B virus infection --- p.9Chapter 1.3.1.3 --- Hepatitis C virus infection --- p.11Chapter 1.3.1.4 --- Hepatitis D virus infection --- p.12Chapter 1.3.1.5 --- Hepatitis E virus infection --- p.12Chapter 1.3.2 --- Cancer of the liver --- p.13Chapter 1.3.2.1 --- Hepatocellular carcinoma --- p.13Chapter 1.3.2.2 --- Cholangiocarcinoma --- p.14Chapter 1.3.2.3 --- Metastatic liver cancer --- p.14Chapter 1.4 --- Conventional treatment of liver disorders --- p.14Chapter 1.5 --- Role of traditional Chinese medicines in hepatoprotective functions --- p.16Chapter 1.5.1 --- Abri Herba (Abrus Cantoniensis Hance) --- p.17Chapter 1.5.2 --- Rhizoma Coptidis (Coptidis chinensis Franch) --- p.18Chapter 1.5.3 --- Fructus Forsythia (Forsythia suspense (Thunb) Vahl) --- p.22Chapter 1.6 --- Molecular studies of hepatoprotective effects of TCMs --- p.26Chapter 1.6.1 --- Roles of detoxofication enzymes in hepatoprotection --- p.27Chapter 1.6.2 --- Studies of growth-related genes in cell cycle control --- p.29Chapter 1.7 --- Aims of project --- p.32Chapter 1.8 --- Application of the project --- p.33Chapter Chapter 2 --- Methods and materials --- p.34Chapter 2.1 --- Screening of traditional Chinese medicines --- p.35Chapter 2.2 --- Preparation of TCMs --- p.35Chapter 2.2.1 --- Preparation of aqueous extracts of TCMs --- p.35Chapter 2.2.2 --- Preparation of active components of TCMs --- p.36Chapter 2.3 --- In vitro assays --- p.40Chapter 2.3.1 --- Cell culture --- p.40Chapter 2.3.2 --- Cytotoxicity test --- p.40Chapter 2.4 --- Screening of expressed gene induced by TCMs --- p.41Chapter 2.4.1 --- RNA preparation --- p.41Chapter 2.4.2 --- cDNA array hybridization --- p.42Chapter 2.4.3 --- Reverse Transcription --- p.43Chapter 2.5 --- Confirmation of expressed genes induced by TCMs --- p.44Chapter 2.5.1 --- Semi-quantitative PCR analysis --- p.44Chapter 2.5.2 --- Northern blot analysis --- p.46Chapter 2.6 --- Studies of effects of TCMs in gene expression --- p.47Chapter 2.6.1 --- Dosage-course study --- p.47Chapter 2.6.2 --- Time-course study --- p.48Chapter Chapter 3 --- Results --- p.50Chapter 3.1 --- "Cytotoxicity test of A.H., R.C. and F.F" --- p.51Chapter 3.2 --- "Molecular screening of expressed gene induced by A.H., R.C., F.F" --- p.58Chapter 3.3 --- Confirmation of expressed gene using semi-quantitative RT- PCR --- p.70Chapter 3.3.1 --- Dosage-course and time-course studies of A.H. using RT- PCR --- p.70Chapter 3.3.2 --- Dosage-course and time-course studies of R.C. using RT- PCR --- p.94Chapter 3.3.3 --- Dosage-course and time-course studies of A.H. using RT- PCR --- p.113Chapter 3.4 --- Confirmation of expressed gene using northern blot anaylsis --- p.118Chapter 3.4.1 --- Dosage-course and time-course studies of effects of A.H. and L- abrine in Northern blot analysis --- p.118Chapter 3.4.2 --- Dosage-course and time-course studies of effects of R.C. and berberine in Northern blot analysis --- p.129Chapter 3.4.3 --- Dosage-course and time-course studies of effects of F.Fin Northern blot analysis --- p.147Chapter Chapter 4 --- Discussion --- p.152Chapter 4.1 --- "Roles of A.H., R.C. and F.F. in treatment and prevention of liver disorders" --- p.153Chapter 4.2 --- "Cytotoxicity effect A.H., R.C., and F.F. in liver cells" --- p.153Chapter 4.3 --- Effects of herbal medicines on the transcription of mRNA in liver cells --- p.155Chapter 4.3.1 --- Effects of treatment of A.H. in liver at transcriptional level … --- p.155Chapter 4.3.2 --- Effects of treatment of R.C. in liver at transcriptional level … --- p.156Chapter 4.3.3 --- Effects of treatment of R.C. in liver at transcriptional level --- p.157Chapter 4.4 --- Comparison of results of RT-PCR and Northern blot analysis --- p.157Chapter 4.4.1 --- Comparison of the effects of time and dosage-course studies of DTD expression induced by A.H. and L-abrine --- p.157Chapter 4.4.2 --- Comparison of the effects of time and dosage-course studies of p21;cip;waf1 expression induced by A.H. and L-abrine --- p.158Chapter 4.4.3 --- Comparison of the effects of time and dosage-course studies of c-myc responsive protein; rcl expression induced by R.C. and berberine --- p.159Chapter 4.4.4 --- Comparison of the effects of time and dosage-course studies of GST Ya expression induced by R.C. and berberine --- p.160Chapter 4.4.5 --- Comparison of the effects of time and dosage-course studies of GST 7-7 expression induced by F.F --- p.160Chapter 4.5 --- Biochemical significance of genes induced by hepatoprotective TCMs --- p.161Chapter 4.5.1 --- Roles of significant expression of detoxifying enzymes induced by TCMs in liver cells --- p.161Chapter 4.5.2 --- Roles of induction of growth-related c-myc responsive protein; rcl in R.C. treated liver cells --- p.167Chapter 4.5.3 --- Roles of increased p21;cip;waf1 expression in A.H. treated liver cells --- p.168Chapter 4.6 --- Conclusion --- p.16