Human papillomavirus type 16 infection in cervical neoplasm: viral load analysis.

Yeung Sze-wan.Thesis (M.Phil.)--Chinese University of Hong Kong, 2003.Includes bibliographical references.Abstracts in English and Chinese.ACKNOWLEDGEMENT --- p.iABSTRACT --- p.iiABBREVIATIONS --- p.viiTABLE OF CONTENTS --- p.ixChapter CHAPTER 1 --- INTRODUCTION --- p.1-1Chapter 1.1 --- Anatomy of the Cervix --- p.1-1Chapter 1.2 --- Histology --- p.1-1Chapter 1.2.1 --- Squamous Epithelium --- p.1-1Chapter 1.2.2 --- The Endocervical Epithelium --- p.1-3Chapter 1.2.3 --- The Squamo-columnar Junction --- p.1-4Chapter 1.2.3.1 --- The Embryology --- p.1-4Chapter 1.2.3.2 --- Definition --- p.1-4Chapter 1.3 --- Human Papillomaviruses (HPVs) --- p.1-6Chapter 1.3.1 --- Structure of the Viruses --- p.1-6Chapter 1.3.2 --- The Nomenclature --- p.1-7Chapter 1.3.3 --- HPVs Genomic Structure and Properties of Gene Products --- p.1-7Chapter 1.3.4 --- Target Tissues --- p.1-8Chapter 1.3.5 --- Role of HPVs in the Carcinogenesis of Lesions --- p.1-9Chapter 1.3.6 --- Risk Groups of HPVs --- p.1-10Chapter 1.4 --- Pathology --- p.1-11Chapter 1.4.1 --- Macroscopic Features --- p.1-11Chapter 1.4.2 --- Symptoms and Diagnosis --- p.1-12Chapter 1.4.3 --- Histopathology --- p.1-13Chapter 1.4.3.1 --- Histopathological Grading of Cervical Intraepithelial Neoplasia --- p.1-19Chapter 1.4.3.2 --- Staging of Cervical Cancer --- p.1-24Chapter 1.5 --- Epidemiology of Cervical Intraepithelial Neoplasia and Cervical Cancer --- p.1-27Chapter 1.5.1 --- Descriptive Epidemiology --- p.1-28Chapter 1.5.2 --- Risk Factors --- p.1-30Chapter 1.6 --- Human Papillomavirus Type 16 --- p.1-42Chapter 1.6.1 --- Role of HPV16 in CIN and Cervical Carcinoma --- p.1-42Chapter 1.6.2 --- Viral Load of HPV 16 in CIN --- p.1-43Chapter 1.6.3 --- HPV 16 Viral Load as a Screening Tool --- p.1-46Chapter 1.7 --- Quantitation of HPV 16 --- p.1-48Chapter 1.7.1 --- Methods in Viral Quantification --- p.1-48Chapter 1.7.2 --- Selection of Methodology --- p.1-51Chapter 1.7.3 --- Correlation of HPV 16 Viral Loading with Severity of Cervical Lesions --- p.1-54Chapter CHAPTER 2 --- AIMS OF STUDY --- p.2-1Chapter CHAPTER 3 --- MATERIALS AND METHODS --- p.3-1Chapter 3.1 --- Materials --- p.3-1Chapter 3.1.1 --- Patients and Specimens --- p.3-1Chapter 3.2 --- Methods --- p.3-3Chapter 3.2.1 --- DNA Extraction --- p.3-3Chapter 3.2.2 --- Polymerase Chain Reaction --- p.3-7Chapter 3.2.3 --- Gel Electrophoresis --- p.3-8Chapter 3.2.4 --- Real-time Quantitation Polymerase Chain Reaction --- p.3-11Chapter 3.2.5 --- Statistical Analysis --- p.3-15Chapter CHAPTER 4 --- RESULTS --- p.4-1Chapter 4.1 --- Grading of Cervical Smears --- p.4-1Chapter 4.2 --- Incidence of HPV 16 Detected in Cervical Smears --- p.4-2Chapter 4.2.1 --- Detection of HPV 16 in Women for Routine Pap Smear --- p.4-2Chapter 4.2.2 --- Detection of HPV 16 in Women for Colposcopic Examination --- p.4-5Chapter 4.3 --- Quantification of HPV 16 by Real-time PCR --- p.4-5Chapter 4.3.1 --- Range of Detection --- p.4-10Chapter 4.3.2 --- Standard Curve --- p.4-12Chapter 4.3.3 --- Reproducibility of Quantitative Real-time PCR --- p.4-17Chapter 4.3.4 --- Sensitivity of Quantitative Real-time PCR --- p.4-17Chapter 4.3.5 --- Detection and Quantification of HPV 16 E6/7 Genes in HPV16 Positive Cervical Scrapes --- p.4-21Chapter 4.4 --- Comparison of HPV 16 Copy Number Detected among Three Lesion Groups --- p.4-22Chapter 4.5 --- Clinical Analysis --- p.4-27Chapter 4.6 --- HPV 16 DNA Copy Number in Lesion Groups --- p.4-28Chapter CHAPTER 5 --- DISCUSSION --- p.5-1Chapter 5.1 --- Selection of Material (Scrapes) --- p.5-1Chapter 5.2 --- Detection of HPV 16 in Cervical Scrapes --- p.5-3Chapter 5.2.1 --- Selection of HPV Type --- p.5-3Chapter 5.2.2 --- Techniques in Detecting HPV Viral Load --- p.5-3Chapter 5.2.2.1 --- Advantages of Quantitative Real-time PCR --- p.5-6Chapter 5.2.2.2 --- Parameters Affecting the Performance of Real-time PCR --- p.5-8Chapter 5.2.3 --- Selection of Detection Sites --- p.5-9Chapter 5.2.4 --- Standard Curve Establishment --- p.5-10Chapter 5.3 --- Comparison between Real-time PCR and Traditional PCR --- p.5-12Chapter 5.4 --- Role of HPV Viral Load in Cervical Neoplasm --- p.5-13Chapter 5.5 --- HPV Infection in Hong Kong Chinese Women --- p.5-17Chapter 5.6 --- Clinical Significance of HPV 16 Viral Load Detected in Cervical Neoplasm --- p.5-18Chapter 5.7 --- Future Prospect --- p.5-20Chapter CHAPTER 6 --- CONCLUSION --- p.6-1REFERENCES --- p.R-