Analysis of flavan-3-ols by capillary electrophoresis.

Lee Wai Hang.Thesis (M.Phil.)--Chinese University of Hong Kong, 2004.Includes bibliographical references (leaves 71-76).Abstracts in English and Chinese.Acknowledgement --- p.iAbstract --- p.iiTable of Contents --- p.vAbbreviations --- p.viiiList of Figures --- p.ixList of Tables --- p.xivChapterChapter 1. --- Introduction --- p.1Chapter 1.1 --- The French Paradox --- p.1Chapter 1.2 --- Flavonoids --- p.2Chapter 1.3 --- Grape seed extract --- p.4Chapter 1.4 --- Instrunmental analysis --- p.7Chapter 1.4.1 --- High Performance Liquid Chromatography --- p.7Chapter 1.4.2 --- Colorimetry --- p.9Chapter 1.5 --- Capillary Electrophoresis --- p.10Chapter 1.5.1 --- Instrunmentation --- p.10Chapter 1.5.2 --- Electroosmotic Flow --- p.11Chapter 1.5.3 --- Electrophoretic mobility --- p.13Chapter 1.6 --- Objective of the study --- p.15Chapter 2. --- Experimental --- p.18Chapter 2.1 --- Reagents and material --- p.18Chapter 2.1.1 --- Reagents --- p.18Chapter 2.1.2 --- Instrunmentation --- p.18Chapter 2.1.3 --- Reference compounds --- p.19Chapter 2.1.4 --- Samples --- p.19Chapter 2.2 --- Selection of solvent for sample preparation --- p.20Chapter 2.3 --- Procedures --- p.21Chapter 2.3.1 --- Preparation of running buffer solution --- p.21Chapter 2.3.2 --- Preparation of standard solution --- p.21Chapter 2.3.3 --- Preparation of sample solution --- p.22Chapter 2.3.4 --- Flushing procedures --- p.22Chapter 3. --- Results and Discussion --- p.24Chapter 3.1 --- Preliminary experiments --- p.24Chapter 3.2 --- Effect of pH --- p.27Chapter 3.3 --- Addition of surfactant --- p.30Chapter 3.4 --- Effect of SDS concentration --- p.35Chapter 3.5 --- Addition of cyclodextrins --- p.39Chapter 3.6 --- Urea --- p.46Chapter 3.7 --- Addition of organic modifier --- p.47Chapter 3.8 --- Effect of borate concentration --- p.49Chapter 3.9 --- Effect of cyclodextrin concentration --- p.53Chapter 3.10 --- Optimized condition --- p.58Chapter 3.11 --- Reproducibility of the method --- p.58Chapter 3.12 --- Quantitative analysis of reference compounds --- p.60Chapter 3.13 --- Application of the CE method in grape seed products --- p.62Chapter 4. --- Conclusion --- p.69References --- p.7