The effects of curcumin on skeletal muscle regeneration.

Tsang Kwai Fan.Thesis submitted in: December 2003.Thesis (M.Phil.)--Chinese University of Hong Kong, 2004.Includes bibliographical references (leaves 85-101).Abstracts in English and Chinese.Abstracts --- p.i摘要 --- p.iiiAcknowledgements --- p.vTable of Abbreviations --- p.viTable of Contents --- p.viiChapter Chapter One: --- Introduction of CurcuminChapter 1.1 --- What is curcumin? --- p.1Chapter 1.2 --- Chemistry of curcumin --- p.2Chapter 1.3 --- History of using turmeric --- p.3Chapter 1.4 --- Curcumin in biological system --- p.4Chapter 1.5 --- Current researches on curcumin --- p.7Chapter 1.6 --- Curcumin Toxicity --- p.13Chapter Chapter Two: --- Introduction of Muscle RegenerationChapter 2.1 --- Reasons for studying muscle regeneration --- p.15Chapter 2.2 --- Skeletal muscle: functions and structure --- p.16Chapter 2.3 --- Muscle healing process --- p.17Chapter 2.4 --- Muscle formation --- p.31Chapter 2.5 --- Current trends in enhancing muscle regeneration --- p.37Chapter Chapter Three: --- MethodologyChapter 3.1 --- Animals --- p.41Chapter 3.2 --- Histology --- p.42Chapter 3.3 --- Measurement of cross-sectional area of muscles --- p.42Chapter 3.4 --- Two-dimensional electrophoresis --- p.43Chapter 3.5 --- Protein identification by matrix-assisted laser desorption time-of-flight (MALDI-TOF) --- p.47Chapter 3.6 --- Cell culture --- p.50Chapter 3.7 --- In vitro cell proliferation assayChapter 3.8 --- In vitro differentiation assay --- p.51Chapter 3.9 --- Transfection and Secreted Alkaline Phosphatase (SEAP) reporter assay --- p.52Chapter Chapter Four: --- ResultsChapter 4.1 --- Effects of curcumin on muscle regeneration --- p.58Chapter 4.2 --- Two-dimensional electrophoresis --- p.59Chapter 4.3 --- Effect of curcumin on myogenic cell proliferation --- p.61Chapter 4.4 --- Effect of curcumin on myogenic cell differentiation --- p.61Chapter 4.5 --- Signal transduction --- p.63Chapter 4.6 --- Legends and table --- p.65Chapter Chapter Five: --- Discussion --- p.77Chapter Chapter Six: --- Conclusions --- p.84References --- p.85Appendices --- p.10