Application of affinity mass sensor based on boronic acid derivatives.

Chow Ka-man.Thesis (M.Phil.)--Chinese University of Hong Kong, 2001.Includes bibliographical references (leaves 52-55).Abstracts in English and Chinese.Chapter 1 --- IntroductionChapter 1.1 --- Chemical sensors --- p.1Chapter 1.2 --- Quartz crystal microbalance --- p.4Chapter 1.3 --- Concept of affinity mass sensor --- p.8Chapter 1.4 --- Film immobilization technologies --- p.9Chapter 1.5 --- Research outlines --- p.13Chapter 2 --- ExperimentalChapter 2.1 --- Sensor fabrication --- p.14Chapter 2.2 --- Flow-through cell --- p.16Chapter 2.3 --- Analysis procedures --- p.19Chapter 2.4 --- Response curve --- p.19Chapter 2.5 --- Experimental setup --- p.21Chapter 3 --- Detection of ascorbic acid by affinity mass sensor based on 3-aminophenylboronic acidChapter 3.1 --- Conventional analytical methods --- p.23Chapter 3.2 --- Research method - affinity mass sensor based on APBA --- p.24Chapter 3.3 --- To locate the binding site in ascorbic acid --- p.25Chapter 3.3.1 --- Steric energy calculated by molecular modeling --- p.26Chapter 3.4 --- Optimization of experimental variables --- p.29Chapter 3.4.1 --- Effect of pH --- p.29Chapter 3.4.2 --- Effect of sample volume --- p.30Chapter 3.4.3 --- Effect of flow velocity --- p.30Chapter 3.5 --- Calibration and Reproducibility --- p.32Chapter 3.6 --- Kinetic analysis --- p.33Chapter 3.7 --- Stability of sensor --- p.37Chapter 3.8 --- Interference studies --- p.37Chapter 3.9 --- Determination of ascorbic acid in real samples --- p.39Chapter 3.9.1 --- Results and Discussion --- p.39Chapter 3.10 --- Comparison with conventional ascorbic acid sensors --- p.42Chapter 3.11 --- Summary --- p.42Chapter 4 --- Boronic acid derivatives for the detection of sugarsChapter 4.1 --- Scope of this work --- p.43Chapter 4.2 --- Results and Discussion --- p.44Chapter 4.3 --- Summary --- p.49Conclusion --- p.50References --- p.52List for tables --- p.56List for figures --- p.57Appendix I --- p.59Appendix II --- p.6