Study of anti-cancer effect of a Trichosanthes sp. extract.

Tang Sze-Wan.Thesis (M.Phil.)--Chinese University of Hong Kong, 2005.Includes bibliographical references (leaves 104-118).Abstracts in English and Chinese.Abstract --- p.iAbstract (Chinese) --- p.iiiAcknownledgement --- p.ivList of Abbreviations --- p.vList of Tables --- p.viiList of Figures --- p.viiiTable of Contents --- p.xiChapter Chapter 1 - --- IntroductionChapter 1.1 --- Trichosanthes spp --- p.1Chapter 1.1.1 --- Use of Trichosanthes --- p.2Chapter 1.1.2 --- Trichosanthin --- p.2Chapter 1.1.3 --- Karasurin --- p.5Chapter 1.1.4 --- Ribosome Inactivating Proteins --- p.6Chapter 1.1.5 --- Immunosuppresion --- p.7Chapter 1.1.6 --- Anti-Cancer Activity --- p.8Chapter 1.1.7 --- Miscellaneous Uses --- p.8Chapter 1.2 --- Cancer --- p.9Chapter 1.2.1 --- Oncogenes --- p.10Chapter 1.2.2 --- Tumor-Suppressor Genes --- p.11Chapter 1.2.3 --- Stability Genes --- p.12Chapter 1.2.4 --- Types of Cancer --- p.13Chapter 1.2.5 --- Cancer Therapy --- p.13Chapter 1.2.6 --- Apoptosis --- p.14Chapter 1.3 --- Chronic Myelogenous Leukemia (CML) --- p.17Chapter 1.3.1 --- Philadelphia Chromosome and BCR-ABL gene --- p.18Chapter 1.3.2 --- Treatment of CML --- p.20Chapter 1.4 --- Dendritic Differentiation of LC976 on K-562 --- p.20Chapter 1.4.1 --- Dendritic Cells --- p.21Chapter 1.4.2 --- Cancer Vaccine Development of Leukemia --- p.22Chapter 1.4.3 --- Dendritic differentiation of K-562 cells --- p.23Chapter 1.5 --- Perspective of the Project --- p.23Chapter Chapter 2 - --- Materials and MethodsChapter 2.1 --- MaterialsChapter 2.1.1 --- Chemicals and Reagents --- p.25Chapter 2.1.2 --- Bioassay Kits --- p.26Chapter 2.1.3 --- Human Cell Lines --- p.26Chapter 2.1.4 --- Lab Wares and Equipments --- p.28Chapter 2.2 --- Extraction of LC9 --- p.76Chapter 2.2.1 --- Chemical Properties of the Lead Compound --- p.28Chapter 2.2.2 --- Crude Extraction of Trichosanthes sp --- p.29Chapter 2.2.3 --- Purification by Reversed-Phase Column --- p.29Chapter 2.2.4 --- Lyophilization and Preparation of LC976 --- p.31Chapter 2.3 --- Anti-Proliferation Effect of LC976 on Human Cell LinesChapter 2.3.1 --- Maintenance of Cell Lines --- p.32Chapter 2.3.2 --- MTT Assay --- p.32Chapter 2.3.3 --- BrdU Cell Proliferation ELISA --- p.34Chapter 2.4 --- Apoptosis Induction on K-5 --- p.62Chapter 2.4.1 --- PI Staining --- p.35Chapter 2.4.2 --- Annexin V-FITC FACS Analysis --- p.36Chapter 2.4.3 --- Caspase Activation --- p.37Chapter 2.5 --- Effect on Normal Human LymphocytesChapter 2.5.1 --- Preparation of Human Normal Lymphocytes --- p.38Chapter 2.5.2 --- MTT Cell Viability Assay --- p.38Chapter 2.5.3 --- PI Staining --- p.39Chapter 2.5.4 --- Annexin V-FITC FACS Analysis --- p.39Chapter Chapter 3 - --- ResultsChapter 3.1 --- Extraction of LC976 --- p.40Chapter 3.2 --- LC976 Inhibited Proliferation of Human Cell LinesChapter 3.2.1 --- MTT Assay --- p.41Chapter 3.2.2 --- BrdU Cell Proliferation ELISA --- p.52Chapter 3.3 --- LC976 Induced Apoptosis in K-562 CellsChapter 3.3.1 --- PI Staining --- p.63Chapter 3.3.2 --- Annexin V-FITC FACS Analysis --- p.70Chapter 3.3.3 --- Caspase Activation --- p.73Chapter 3.4 --- Effect on Normal Human LymphocytesChapter 3.4.1 --- MTT Cell Viability Assay --- p.76Chapter 3.4.2 --- PI Staining --- p.78Chapter 3.4.3 --- Annexin V-FITC FACS Analysis --- p.82Chapter Chapter 4 - --- DiscussionChapter 4.1 --- Extraction of LC976 --- p.85Chapter 4.2 --- LC976 Inhibited Proliferation of Human Cell LinesChapter 4.2.1 --- MTT Assay --- p.86Chapter 4.2.2 --- BrdU Cell Proliferation ELISA --- p.88Chapter 4.3 --- LC976 induced Apoptosis in K-562 CellsChapter 4.3.1 --- PI Staining --- p.90Chapter 4.3.2 --- Annexin V-FITC Analysis --- p.95Chapter 4.3.3 --- Caspase Activation --- p.96Chapter 4.4 --- Effect on Normal Human LymphocytesChapter 4.4.1 --- MTT Cell Viability Assay --- p.98Chapter 4.4.2 --- PI Staining --- p.99Chapter 4.4.3 --- Annexin V-FITC FACS Analysis --- p.100Chapter 4.5 --- Conclusion --- p.103Reference --- p.10