Genetic characterization of BAC clones from a Senegalese sole BAC library containing genes of interest in aquaculture

Senegalese sole (Solea senegalensis, Kaup 1858), is one of the most economically valued species in aquaculture. One of the main troubles found in the Senegalese sole culture were the low growth rates in juveniles and the greater susceptibility to disease. The study of genomes belonging to so high value species is essential to improve and optimize its culture. In that sense, knowing the location of genes of interest in the Senegalese sole chromosomes facilitates the construction of linkage maps, and it is useful in evolution studies beca use the rearrangements that occur in the course of evolution can be deduced through the analysis of the relative positions of these genes in related organisms. This work is a first approach in the development of a physical map of S. senegalensis. In order to gain that objective, the work started from a genomic library cloned in vectors capable of accommodating large fragments, in our case, Bacterial Artificial Chromosomes (BAC).Using FISH we have located BAC clones holding genes involved in the innate immune system and metamorphosis in the chromosomes of Senegalese sole. El lenguado senegalés (Solea senegalensis, Kaup 1858), es una de las especies más valoradas económicamente en la acuicultura. Los principales problemas con los que se encuentra el cultivo del lenguado senegalés son las bajas tasas de crecimiento de los juveniles y la mayor susceptibilidad a las enfermedades. El estudio de los genomas pertenecientes a especies de valor tan alto es esencial para mejorar y optimizar su cultivo. Este trabajo es una primera aproximación en el desarrollo de un mapa físico de S. senegalensis.