Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
- Moreira,Keila Aparecida
- Diogo,Margarida
- Prazeres,Duarte Miguel
- Lima Filho,José Luiz de
- Porto,Ana Lúcia Figueiredo
DOIAbstract
This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA
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