Produ??o de biomassa microalgal de Desmodesmus sp. utilizando hidrolisado hemicelul?sico proveniente do baga?o de cana-de-a??car como fonte de carbono visando a obten??o de biocombust?veis de terceira gera??o

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2020-03-10T17:02:06Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) marina_lemos_sartori.pdf: 1676632 bytes, checksum: 4a042ea38450cd2049614dabb4a12dda (MD5)Approved for entry into archive by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2020-03-11T15:40:43Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) marina_lemos_sartori.pdf: 1676632 bytes, checksum: 4a042ea38450cd2049614dabb4a12dda (MD5)Made available in DSpace on 2020-03-11T15:40:43Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) marina_lemos_sartori.pdf: 1676632 bytes, checksum: 4a042ea38450cd2049614dabb4a12dda (MD5) Previous issue date: 2019As microalgas t?m sido apresentadas como biomassas com larga vantagem para a produ??o de biocombust?veis quando comparadas com as biomassas agr?colas ou florestais. Foi avaliado o aproveitamento do hidrolisado hemicelul?sico proveniente do pr?-tratamento ?cido do baga?o de cana-de-a??car, um dos mais abundantes res?duos agroindustriais gerados no Brasil, como fonte de carbono para a produ??o de biomassa microalgal. O hidrolisado hemicelul?sico ? um subproduto da ind?stria do etanol de segunda gera??o e apresenta grande quantidade de a??cares livres, principalmente a xilose. Neste sentido, estudou-se a capacidade da linhagem unialgal de Desmodesmus sp. em utilizar a xilose presente no hidrolisado hemicelul?sico e acumular ?leo, carboidratos e prote?nas. As condi??es autotr?fica, mixotr?fica e heterotr?fica foram testadas e comparadas. A linhagem de Desmodesmus sp. estudada foi capaz de metabolizar xilose em meio sint?tico e, tamb?m, a xilose contida no hidrolisado hemicelul?sico. Os maiores teores de prote?na, carboidrato e ?leo, expressos em peso seco, foram 65,48?4,87%, 29,61?3,47% e 15,81?0,15%, respectivamente. Os dados de produtividade registrados sugerem que Desmodesmus sp. apresenta maior capacidade para a produ??o de prote?nas e carboidratos na condi??o de cultivo mixotr?fico. A partir das caracter?sticas observadas da microalga Desmodesmus sp. foi poss?vel constatar que essa apresenta um melhor perfil para a produ??o de bioetanol de terceira gera??o.Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2019.Microalgae have been presented as biomass with a great advantage for the production of biofuels when compared to agricultural or forest biomasses. The use of the hemicellulosic hydrolyzate from the acid pretreatment of sugarcane bagasse, one of the most abundant agroindustrial residues generated in Brazil, was evaluated as a source of carbon for the production of microalgal biomass. The hemicellulosic hydrolyzate is a byproduct of the second generation ethanol industry and has a large amount of free sugars, mainly xylose. In this sense, was studied the capacity of the unialgal strain of Desmodesmus sp. in using the xylose present in the hemicellulosic hydrolyzate and accumulating oil, carbohydrates and proteins. The autotrophic, mixotrophic and heterotrophic conditions were tested and compared. The lineage of Desmodesmus sp. was able to metabolize xylose in synthetic medium and also the xylose contained in the hemicellulosic hydrolyzate. The best protein, carbohydrate and oil contents, expressed in dry weight, were 65.48 ? 4.87%, 29.61 ? 3.47% and 15.81 ? 0.15%, respectively. The productivity data presented suggest that Desmodesmus sp. shows higher capacity for the production of proteins and carbohydrates in the condition of mixotrophic culture using the free sugars present in the hemicellulosic hydrolyzate. From the observed characteristics of the microalga Desmodesmus sp. it was possible to verify that this presents a better profile for the production of third generation bioethanol