Biological control of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) with entomopathogenic fungus of Beauveria bassiana, Metarhizium anisopliae and Isaria sp

Data de aprova??o ausente.Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2016-12-19T14:23:22Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) thatiane_aparecida_evangelista.pdf: 8873850 bytes, checksum: 463ebcb9203efdde8e7dcd55c92e226a (MD5)Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-12-19T16:52:04Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) thatiane_aparecida_evangelista.pdf: 8873850 bytes, checksum: 463ebcb9203efdde8e7dcd55c92e226a (MD5)Made available in DSpace on 2016-12-19T16:52:04Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) thatiane_aparecida_evangelista.pdf: 8873850 bytes, checksum: 463ebcb9203efdde8e7dcd55c92e226a (MD5) Previous issue date: 2016Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)Thaumastocoris peregrinus foi detectado no Brasil em 2008 e se disseminou pelos plantios florestais brasileiros de tal forma que, rapidamente, atingiu o n?vel de dano econ?mico e causou muitos preju?zos aos produtores. Por n?o haver nenhum m?todo de controle definido, utilizaram-se de pulveriza??es com inseticidas qu?micos para o controle desse inseto. No entanto, devido ao processo de certifica??o florestal, esse m?todo de controle foi rapidamente proibido. Assim, o controle biol?gico tem sido apontado como o mais adequado ao controle at? que se consiga gen?tipos resistentes. O uso de fungos entomopatog?nicos ? apontado como uma das melhores maneiras de controle do percevejo bronzeado devido o seu baixo impacto ambiental, dentre outras vantagens. Logo, objetivou-se selecionar isolados de Metarhizium anisopliae, Isaria fumosorosea, Isaria farinosa e Beauveria bassiana com potencial para o controle dessa praga. Em condi??es controladas testaram-se diferentes concentra??es (controle, 104, 106 e 108 esporos por ml) em indiv?duos adultos de T. peregrinus, em cinco repeti??es. Os indiv?duos foram mantidos em placas de Petri sobre partes de folhas (4 cm de di?metro) previamente inoculadas com a suspens?o de esporos. Os tratamentos foram avaliados diariamente quanto a mortalidade e os dados submetidos ? an?lise estat?stica. Considerou-se patog?nico o isolado cujo percentual de mortalidade sobre T. peregrinus foi igual ou superior a 80%. Na maior concentra??o todos os isolados de B. bassiana registraram mortalidade superior a 85%. No entanto, pelos testes de m?dia, alguns isolados se destacaram, tais como IBCB63 de Beauveria bassiana, IBCB159 de Metarhizium anisopliae, IBCB130 de Isaria fumosorosea e CG195 de Isaria farinosa. Assim constatou-se que, em laborat?rio, ? poss?vel o controle eficaz dessa praga por meio do uso de fungos entomopatog?nicos e que existem diferentes isolados, dentre as esp?cies testadas, com potencial de uso. Essa diversidade de isolados aptos ao controle ? importante devido ao processo de sele??o natural que leva ao aparecimento de insetos resistentes. Em um futuro uso, esses isolados poder?o ser intercalados e inibir ou retardar a ocorr?ncia desses indiv?duos.Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016.Thaumastocoris peregrinus was detected in Brazil in 2008 and spread by Brazilian forest plantations such a way that quickly reached the level of economic damage and caused many losses to producers. Because there is no defined control method, pulverizations with chemical insecticides were used to control this insect. However, due to the forest certification process, this method of control was quickly banned. Thus, biological control has been touted as the most suitable control method, until the identification of resistant genotypes. The use of entomopathogenic fungi is considered one of the best ways to control the bronze bug due to its low environmental impact, among other advantages. Therefore, this study aimed to select isolates of Metarhizium anisopliae, Isaria fumosorosea, Isaria farinosa and Beauveria bassiana with potential to control this pest. Under controlled conditions, different concentrations were tested (control, 104, 106 and 108 spores per ml) in adults of T. peregrinus in five replicates. The individuals were kept on Petri dishes over parts of leaves (diameter 4 cm) that were previously inoculated with the spore suspension. The treatments were evaluated daily for mortality and the data analyzed statistically. The isolate whose mortality percentage of T. peregrinus was equal or higher than 80% was considered pathogenic. At higher concentration, all the isolates of B. bassiana reported mortalities greater than 85%. However, the mean tests demonstrated that some isolates stood out, such as IBCB63 of Beauveria bassiana, IBCB159 of Metarhizium anisopliae, IBCB130 of Isaria fumosorosea and CG195 of Isaria farinosa. It was found that in laboratorial conditions, it is possible to control effectively this pest through the use of entomopathogenic fungi, and that there are different isolates with potential use among the species tested. This diversity of isolates able to the control it is important because of the natural selection process that leads to the onset of resistant insects. In a further use, these isolates can be interleaved and inhibit or delay the occurrence of these individuals