Role of immune complexes from pacients with different clinical forms of Schistosomiasis in the modulation of in vitro granuloma reaction.

Submitted by Karine Ribeiro (karinelg.ribeiro@gmail.com) on 2014-11-19T18:11:23Z No. of bitstreams: 1 ARTIGO_RoleImmuneComplexes.pdf: 70468 bytes, checksum: 3fcf88b171c67b5b207143ed9a69b72f (MD5)Rejected by Oliveira Flávia (flavia@sisbin.ufop.br), reason: Só a primeira palavra do título e nomes próprios com inicial maiúscula. on 2014-11-19T18:21:29Z (GMT)Submitted by Karine Ribeiro (karinelg.ribeiro@gmail.com) on 2014-11-19T18:44:47Z No. of bitstreams: 1 ARTIGO_RoleImmuneComplexes.pdf: 70468 bytes, checksum: 3fcf88b171c67b5b207143ed9a69b72f (MD5)Approved for entry into archive by Oliveira Flávia (flavia@sisbin.ufop.br) on 2014-11-19T19:42:33Z (GMT) No. of bitstreams: 1 ARTIGO_RoleImmuneComplexes.pdf: 70468 bytes, checksum: 3fcf88b171c67b5b207143ed9a69b72f (MD5)Made available in DSpace on 2014-11-20T17:06:37Z (GMT). No. of bitstreams: 1 ARTIGO_RoleImmuneComplexes.pdf: 70468 bytes, checksum: 3fcf88b171c67b5b207143ed9a69b72f (MD5) Previous issue date: 1997Schistosomiasis is a disease whose pathology is strongly related to the granulomatous reaction formed around parasite eggs trapped in host tissues. Studies have shown that the chronic intestinal form (INT) of this infection is associated with a variety of immunoregulatory mechanisms which lead to a diminished granulomatous reaction. Using an in vitro model of granuloma reaction, we show that immune complexes (IC) isolated from sera of INT patients are able to reduce granulomatous reaction developed by peripheral blood mononuclear cells (PBMC) from acute (AC), INT and hepatosplenic (HE) patients to soluble egg antigen (SEA)-conjugated polyacrylamide beads (PB-SEA). This inhibitory activity is also observed in cell proliferation assay of PBMC from INT and HE patients stimulated with SEA and adult worm antigen (SWAP). Furthermore, IC isolated from sera of patients with different clinical forms of the disease are also able to suppress INT patients PBMC reactivity. Therefore, our results show that circulating IC present in sera of patients with different clinical forms of schistosomiasis may downregulate PBMC reactivity to parasite antigens resulting in a diminished granuloma reaction to parasite eggs