Ação da fototerapia na cicatrização de queimaduras em pele: Análise clínica e histológica

Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2019-10-24T16:56:45Z No. of bitstreams: 1 PDF - José de Alencar Fernandes Neto.pdf: 4450669 bytes, checksum: d1221c3cdd2d83a08b80690df54cf04c (MD5)Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2019-10-31T11:49:07Z (GMT) No. of bitstreams: 1 PDF - José de Alencar Fernandes Neto.pdf: 4450669 bytes, checksum: d1221c3cdd2d83a08b80690df54cf04c (MD5)Made available in DSpace on 2019-10-31T11:49:07Z (GMT). No. of bitstreams: 1 PDF - José de Alencar Fernandes Neto.pdf: 4450669 bytes, checksum: d1221c3cdd2d83a08b80690df54cf04c (MD5) Previous issue date: 2017-06-19Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESBurns are considered a serious public health problem worldwide. Among these lesions, third degree burns are considered the most serious ones, since they cause the destruction of all layers of the skin, which can also reach muscles, ligaments, tendons, and bones. Currently, among the new studied therapies that aim to accelerate and optimize the healing process of these injuries, there is the phototherapy with the use of Light Emitting Diode (LED) at different wavelengths. The objective of this study was to evaluate the effects of red and blue LED photobiomodulation on the healing of third degree burns in rat skin, through clinical and histological parameters. 60 Wistar male rats were randomly divided into three groups: control (CTR) (n = 20), red LED (RED) (n = 20) and blue LED (BLUE), with subgroups (n = 5) for each time of euthanasia (7, 14, 21 and 28 days). The red (630 ± 10nm, 300 mW, 9 J/cm2 per point, 30 seconds) and blue LED applications (470 nm, 1W, 12.5 J/cm2 per point, 28 seconds) were performed daily in contact with four points of the wounds (total: 36 J/cm2 in the RED and 50 J/cm2 in the BLUE), except on the day of euthanasia. The food consumption of the animals was measured every two days in the 3 groups, with the aid of an analytical balance. After euthanasia of the animals at the pre-established times, the Wound Retention Index (WRI) and removal of the specimen were performed and the sections were stained in Hematoxylin-Eosin (HE) and Masson's Trichrome (MT). At 7 days, it was observed that the WRI of the CTR was greater than the RED (p = 0.01) and there were no statistically significant differences between the BLUE and CTR groups (p> 0.05) in all evaluated periods. The Angiogenic Index (AI) of CTR was lower when compared to RED (p = 0.02) and BLUE (p = 0.01) in 7 days, and higher when compared to RED in 21 days (0.04). The CTR animals consumed less food than the RED ones at 7, 14 and 28 days (p 0,05) em todos os períodos avaliados. O Índice Angiogênico (IA) do CTR foi menor quando comparado ao VERM (p=0,02) e AZUL (p=0,01) em 7 dias, e maior quando comparado ao VERM em 21 dias (0,04). Os animais do CTR consumiram menos ração que o VERM em 7, 14 e 28 dias (p<0,05) e que o AZUL em 14, 21 e 28 dias (p