Add to ORKGAdvances in molecular biology using different types of cells, vectors, and novel methods of DNA delivery, are paving the way for the development of reproducible bioprocesses that yield large amounts of recombinant proteins. A new mammalian cell expression technology has been applied in this study. The FreeStyle TM MAX Expression System offers the advantages of a completely animal origin-free, large-scale transient transfection system that produces high quantity recombinant proteins. Different combinations of plasmid DNAs and transfection reagents with FreeStyle TM CHO-S and FreeStyleTM 293-F cells were tested in order to identify the combination yielding optimal transfection efficiencies. The use of different culture systems, such as shake ...
The expansion of the biologics pipeline depends on the identification of candidate proteins for clin...
The application of transient transfection to the production of recombinant proteins in mammalian cel...
Cell expansion, gene transfer and protein production were all executed with a single serum-free, ani...
This is the first report of two successful 100 l scale transienttransfections in a standard stirred ...
expression, transient transfection This is the first report of two successful 100 l scale transient ...
Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been a...
Recombinant protein-based biologics are a rapidly growing segment of the pharmaceutical marketplace....
Transient gene expression (TGE) in mammalian cells at the reactor scale is becoming increasingly imp...
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines;...
Recent advances in genomics, proteomics, and structural biology raised the general need for signific...
Recombinant proteins are important for biomedical research and for the treatment of human disease. T...
To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have ...
The expansion of the biologics pipeline depends on the identification of candidate proteins for clin...
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines;...
AbstractTraditional mammalian expression systems rely on the time-consuming generation of stable cel...
The expansion of the biologics pipeline depends on the identification of candidate proteins for clin...
The application of transient transfection to the production of recombinant proteins in mammalian cel...
Cell expansion, gene transfer and protein production were all executed with a single serum-free, ani...
This is the first report of two successful 100 l scale transienttransfections in a standard stirred ...
expression, transient transfection This is the first report of two successful 100 l scale transient ...
Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been a...
Recombinant protein-based biologics are a rapidly growing segment of the pharmaceutical marketplace....
Transient gene expression (TGE) in mammalian cells at the reactor scale is becoming increasingly imp...
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines;...
Recent advances in genomics, proteomics, and structural biology raised the general need for signific...
Recombinant proteins are important for biomedical research and for the treatment of human disease. T...
To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have ...
The expansion of the biologics pipeline depends on the identification of candidate proteins for clin...
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines;...
AbstractTraditional mammalian expression systems rely on the time-consuming generation of stable cel...
The expansion of the biologics pipeline depends on the identification of candidate proteins for clin...
The application of transient transfection to the production of recombinant proteins in mammalian cel...
Cell expansion, gene transfer and protein production were all executed with a single serum-free, ani...