New methods are needed to reduce dynamic blebbing which inhibits cell attachment and survival during passaging of pluripotent stem cells. We tested the hypothesis that activation of the P2X7 receptor by extracellular ATP during passaging initiates dynamic blebbing. The P2X7 receptor was present in human embryonic stem cells (hESC), but not in differentiating cells. Extracellular ATP concentrations were 14× higher in medium during passaging. Addition of ATP to culture medium prolonged dynamic blebbing and inhibited attachment. Inhibition of P2X7 by specific drugs or by siRNA significantly reduced dynamic blebbing and improved cell attachment. When cells were incubated in calcium chelators (EGTA or BAPTA), blebbing decreased and attachment im...
none10siRecent evidence indicates that extracellular adenosine triphosphate (eATP), as a major media...
Identifying the signaling mechanisms that regulate adult neurogenesis is essential to understanding ...
The P2X7 receptor is an ATP gated ion channel, that is endowed with a strong pro-apoptotic activity ...
New methods are needed to reduce dynamic blebbing which inhibits cell attachment and survival during...
Dynamic blebs are membrane protrusions on the surface of healthy cells that function in cell divisio...
The P2X7 ATP receptor mediates the cytotoxic effect of extracellular ATP. P2X7-dependent cell death ...
Extracellular nucleotides, released in response to mechanical or inflammatory stimuli, signal throug...
AbstractThe growth of a pluripotent embryonic stem (ES) cell population is dependent on cell surviva...
BACKGROUND: Novel developmental functions have been attributed to the P2X7 receptor (P2X7R) includin...
Background: Novel developmental functions have been attributed to the P2X7 receptor (P2X7R) includin...
AbstractExtracellular ATP induces cation fluxes in and impairs the growth of murine erythroleukemia ...
Contains fulltext : 89873.pdf (publisher's version ) (Closed access)Extracellular ...
High concentrations of ATP induce membrane blebbing. However, the underlying mechanism involved in e...
P2X7 is the largest member of the P2X subfamily of purinergic receptors. A typical feature is the ca...
P2X7 is the largest member of the P2X subfamily of purinergic receptors. A typical feature is the ca...
none10siRecent evidence indicates that extracellular adenosine triphosphate (eATP), as a major media...
Identifying the signaling mechanisms that regulate adult neurogenesis is essential to understanding ...
The P2X7 receptor is an ATP gated ion channel, that is endowed with a strong pro-apoptotic activity ...
New methods are needed to reduce dynamic blebbing which inhibits cell attachment and survival during...
Dynamic blebs are membrane protrusions on the surface of healthy cells that function in cell divisio...
The P2X7 ATP receptor mediates the cytotoxic effect of extracellular ATP. P2X7-dependent cell death ...
Extracellular nucleotides, released in response to mechanical or inflammatory stimuli, signal throug...
AbstractThe growth of a pluripotent embryonic stem (ES) cell population is dependent on cell surviva...
BACKGROUND: Novel developmental functions have been attributed to the P2X7 receptor (P2X7R) includin...
Background: Novel developmental functions have been attributed to the P2X7 receptor (P2X7R) includin...
AbstractExtracellular ATP induces cation fluxes in and impairs the growth of murine erythroleukemia ...
Contains fulltext : 89873.pdf (publisher's version ) (Closed access)Extracellular ...
High concentrations of ATP induce membrane blebbing. However, the underlying mechanism involved in e...
P2X7 is the largest member of the P2X subfamily of purinergic receptors. A typical feature is the ca...
P2X7 is the largest member of the P2X subfamily of purinergic receptors. A typical feature is the ca...
none10siRecent evidence indicates that extracellular adenosine triphosphate (eATP), as a major media...
Identifying the signaling mechanisms that regulate adult neurogenesis is essential to understanding ...
The P2X7 receptor is an ATP gated ion channel, that is endowed with a strong pro-apoptotic activity ...