Add to ORKGWe describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs
A type of retroviral gene trap vectors has been developed that can induce conditional mutations in m...
A major challenge of the postgenomic era is the functional characterization of every single gene wi...
SummaryAll somatic mammalian cells carry two copies of chromosomes (diploidy), whereas organisms wit...
We describe a method for the highly efficient and precise targeted modification of gene trap loci in...
Embryonic stem cell (ESC) differentiation is a useful means by which to produce large quantities of ...
We describe here a resource of mouse embryonic stem (ES) cell lines that contain gene trap insertion...
We describe here a resource of mouse embryonic stem (ES) cell lines that contain gene trap insertion...
Gene targeting in ES cells is extensively used to generate designed mouse mutants and to study gene ...
Conditional inactivation of individual genes in mice using site-specific recombinases is an extremel...
High-throughput gene trapping is a random approach for inducing insertional mutations across the mou...
In an effort to make transgenesis more flexible and reproducible, we developed a system based on nov...
Gene targeting in mouse embryonic stem (ES) cells can be used to generate single gene mutations or d...
Gene targeting in ES cells is extensively used to generate designed mouse mutants and to study gene ...
High-throughput gene trapping is a random approach for inducing insertional mutations across the mou...
A type of retroviral gene trap vectors has been developed that can induce conditional mutations in m...
A type of retroviral gene trap vectors has been developed that can induce conditional mutations in m...
A major challenge of the postgenomic era is the functional characterization of every single gene wi...
SummaryAll somatic mammalian cells carry two copies of chromosomes (diploidy), whereas organisms wit...
We describe a method for the highly efficient and precise targeted modification of gene trap loci in...
Embryonic stem cell (ESC) differentiation is a useful means by which to produce large quantities of ...
We describe here a resource of mouse embryonic stem (ES) cell lines that contain gene trap insertion...
We describe here a resource of mouse embryonic stem (ES) cell lines that contain gene trap insertion...
Gene targeting in ES cells is extensively used to generate designed mouse mutants and to study gene ...
Conditional inactivation of individual genes in mice using site-specific recombinases is an extremel...
High-throughput gene trapping is a random approach for inducing insertional mutations across the mou...
In an effort to make transgenesis more flexible and reproducible, we developed a system based on nov...
Gene targeting in mouse embryonic stem (ES) cells can be used to generate single gene mutations or d...
Gene targeting in ES cells is extensively used to generate designed mouse mutants and to study gene ...
High-throughput gene trapping is a random approach for inducing insertional mutations across the mou...
A type of retroviral gene trap vectors has been developed that can induce conditional mutations in m...
A type of retroviral gene trap vectors has been developed that can induce conditional mutations in m...
A major challenge of the postgenomic era is the functional characterization of every single gene wi...
SummaryAll somatic mammalian cells carry two copies of chromosomes (diploidy), whereas organisms wit...