Double-stranded DNA (dsDNA) cleavage by Cas9 is a hallmark of type II CRISPR-Cas immune systems. Cas9-guide RNA complexes recognize 20-base-pair sequences in DNA and generate a site-specific double-strand break, a robust activity harnessed for genome editing. DNA recognition by all studied Cas9 enzymes requires a protospacer adjacent motif (PAM) next to the target site. We show that Cas9 enzymes from evolutionarily divergent bacteria can recognize and cleave single-stranded DNA (ssDNA) by an RNA-guided, PAM-independent recognition mechanism. Comparative analysis shows that in contrast to the type II-A S. pyogenes Cas9 that is widely used for genome engineering, the smaller type II-C Cas9 proteins have limited dsDNA binding and unwinding act...
The CRISPR-Cas-derived RNA-guided Cas9 endonuclease is the key element of an emerging promising tech...
Bacteria and archaea have evolved multiple defense pathways for protection from invading viruses and...
The CRISPR-Cas revolution is taking place in virtually all fields of life sciences. Harnessing DNA c...
Double-stranded DNA (dsDNA) cleavage by Cas9 is a hallmark of type II CRISPR-Cas immune systems. Cas...
Double-stranded DNA (dsDNA) binding and cleavage by Cas9 is a hallmark of type II CRISPR-Cas bacteri...
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an ...
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an ...
The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA-DNA complementari...
The CRISPR-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA:DNA base-pairing to ta...
The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA:DNA complementari...
The ongoing battle between bacteria and phage has influenced the evolution of complex defense pathwa...
Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave fo...
The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against in...
The CRISPR-Cas-derived RNA-guided Cas9 endonuclease is the key element of an emerging promising tech...
In bacterial defense and genome editing applications, the CRISPR-associated protein Cas9 searches mi...
The CRISPR-Cas-derived RNA-guided Cas9 endonuclease is the key element of an emerging promising tech...
Bacteria and archaea have evolved multiple defense pathways for protection from invading viruses and...
The CRISPR-Cas revolution is taking place in virtually all fields of life sciences. Harnessing DNA c...
Double-stranded DNA (dsDNA) cleavage by Cas9 is a hallmark of type II CRISPR-Cas immune systems. Cas...
Double-stranded DNA (dsDNA) binding and cleavage by Cas9 is a hallmark of type II CRISPR-Cas bacteri...
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an ...
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an ...
The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA-DNA complementari...
The CRISPR-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA:DNA base-pairing to ta...
The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA:DNA complementari...
The ongoing battle between bacteria and phage has influenced the evolution of complex defense pathwa...
Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave fo...
The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against in...
The CRISPR-Cas-derived RNA-guided Cas9 endonuclease is the key element of an emerging promising tech...
In bacterial defense and genome editing applications, the CRISPR-associated protein Cas9 searches mi...
The CRISPR-Cas-derived RNA-guided Cas9 endonuclease is the key element of an emerging promising tech...
Bacteria and archaea have evolved multiple defense pathways for protection from invading viruses and...
The CRISPR-Cas revolution is taking place in virtually all fields of life sciences. Harnessing DNA c...