Add to ORKGInfrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology
Abstract Photolabeling of intracellular molecules is an invaluable approach to studying various dyna...
Abstract We recently converted the GAF domain of NpR3784 cyanobacteriochrome into near-infrared (NIR...
Use of fluorescent proteins to study in vivo processes in mammals requires near-infrared (NIR) bioma...
© 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. Inf...
International audienceFluorescent proteins (FPs) are powerful tools for cell and molecular biology. ...
Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monomeric versions of these ...
International audienceInfrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monome...
Near-infrared fluorescent proteins are in high demand for in vivo imaging. We developed four spectra...
Brighter near-infrared (NIR) fluorescent proteins (FPs) are required for multicolor microscopy and d...
textIn 2008 the Nobel Prize in Chemistry was awarded to the scientists who revolutionized biomedical...
To visualize specific proteins in living cells, cell biologists have fused the green fluorescent pro...
AbstractGenetically encoded fluorescent labels, such as green fluorescent protein, make it possible ...
Fluorescent proteins have proven to be excellent tools for live-cell imaging studies. In addition to...
SummaryNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs) a...
Bright monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags f...
Abstract Photolabeling of intracellular molecules is an invaluable approach to studying various dyna...
Abstract We recently converted the GAF domain of NpR3784 cyanobacteriochrome into near-infrared (NIR...
Use of fluorescent proteins to study in vivo processes in mammals requires near-infrared (NIR) bioma...
© 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. Inf...
International audienceFluorescent proteins (FPs) are powerful tools for cell and molecular biology. ...
Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monomeric versions of these ...
International audienceInfrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monome...
Near-infrared fluorescent proteins are in high demand for in vivo imaging. We developed four spectra...
Brighter near-infrared (NIR) fluorescent proteins (FPs) are required for multicolor microscopy and d...
textIn 2008 the Nobel Prize in Chemistry was awarded to the scientists who revolutionized biomedical...
To visualize specific proteins in living cells, cell biologists have fused the green fluorescent pro...
AbstractGenetically encoded fluorescent labels, such as green fluorescent protein, make it possible ...
Fluorescent proteins have proven to be excellent tools for live-cell imaging studies. In addition to...
SummaryNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs) a...
Bright monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags f...
Abstract Photolabeling of intracellular molecules is an invaluable approach to studying various dyna...
Abstract We recently converted the GAF domain of NpR3784 cyanobacteriochrome into near-infrared (NIR...
Use of fluorescent proteins to study in vivo processes in mammals requires near-infrared (NIR) bioma...