A two-step templated, ribosomal biosynthesis/printing method for the fabrication of protein microarrays for surface plasmon resonance imaging (SPRI) measurements is demonstrated. In the first step, a sixteen component microarray of proteins is created in microwells by cell free on chip protein synthesis; each microwell contains both an in vitro transcription and translation (IVTT) solution and 350 femtomoles of a specific DNA template sequence that together are used to create approximately 40 picomoles of a specific hexahistidine-tagged protein. In the second step, the protein microwell array is used to contact print one or more protein microarrays onto nitrilotriacetic acid (NTA)-functionalized gold thin film SPRI chips for real-time SPRI ...
International audienceIn the past few years, protein and peptide microarrays have shown a great pote...
[[abstract]]Microarrays simultaneously screen tens to thousands of biosamples to observe biochemical...
RNA microarrays were created on chemically modified gold surfaces using a novel surface ligation met...
Protein microarrays are fabricated from double-stranded DNA (dsDNA) microarrays by a one-step, multi...
This dissertation is the culmination of work toward the development of protein microarrays for surfa...
Microarrays of RNA aptamers are fabricated in a one-step, multiplexed enzymatic synthesis on gold th...
The sensitivity and selectivity of surface plasmon resonance imaging (SPRI) biosensing with nucleic ...
A simple method is presented for patterning of protein antigens at a gold surface for use in surface...
Here we introduce microelectrospotting as a new approach for preparation of protein-selective molecu...
Advances in lithographic approaches to fabricating bio-microarrays have been extensively explored ov...
International audienceThe recent advances in microtechnologies have shown the interest of developing...
Surface plasmon resonance imaging (SPR) is a well-established label-free detection technique for rea...
The surface-sensitive optical technique of surface plasmon resonance (SPR) imaging is an ideal metho...
[[abstract]]A novel method of protein array immobilization, using micro stamps to pick up proteins f...
Since the last decade, there is a growing need for patterned biomolecules for various applications r...
International audienceIn the past few years, protein and peptide microarrays have shown a great pote...
[[abstract]]Microarrays simultaneously screen tens to thousands of biosamples to observe biochemical...
RNA microarrays were created on chemically modified gold surfaces using a novel surface ligation met...
Protein microarrays are fabricated from double-stranded DNA (dsDNA) microarrays by a one-step, multi...
This dissertation is the culmination of work toward the development of protein microarrays for surfa...
Microarrays of RNA aptamers are fabricated in a one-step, multiplexed enzymatic synthesis on gold th...
The sensitivity and selectivity of surface plasmon resonance imaging (SPRI) biosensing with nucleic ...
A simple method is presented for patterning of protein antigens at a gold surface for use in surface...
Here we introduce microelectrospotting as a new approach for preparation of protein-selective molecu...
Advances in lithographic approaches to fabricating bio-microarrays have been extensively explored ov...
International audienceThe recent advances in microtechnologies have shown the interest of developing...
Surface plasmon resonance imaging (SPR) is a well-established label-free detection technique for rea...
The surface-sensitive optical technique of surface plasmon resonance (SPR) imaging is an ideal metho...
[[abstract]]A novel method of protein array immobilization, using micro stamps to pick up proteins f...
Since the last decade, there is a growing need for patterned biomolecules for various applications r...
International audienceIn the past few years, protein and peptide microarrays have shown a great pote...
[[abstract]]Microarrays simultaneously screen tens to thousands of biosamples to observe biochemical...
RNA microarrays were created on chemically modified gold surfaces using a novel surface ligation met...