Human platelet ALOX12 (hALOX12 or h12-LOX) has been implicated in a variety of human diseases. The present study investigates the active site of hALOX12 to more thoroughly understand how it positions the substrate and achieves nearly perfect regio- and stereospecificities (i.e., 100 ± 5% of the 12(S)-hydroperoxide product), utilizing site-directed mutagenesis. Specifically, we have determined that Arg402 is not as important in substrate binding as previously seen for hALOX15 but that His596 may play a role in anchoring the carboxy terminal of the arachidonic acid during catalysis. In addition, Phe414 creates a π-stacking interaction with a double bond of arachidonic acid (Δ11), and Ala417/Val418 define the bottom of the cavity. However, the...
SummaryLipoxygenases are critical enzymes in the biosynthesis of families of bioactive lipids includ...
ABSTRACT: 15-Lipoxygenases are lipid-peroxidizing enzymes which have been implicated in the pathogen...
Lipoxygenases (LOX) play critical roles in mammalian biology in the generation of potent lipid media...
Human platelet ALOX12 (hALOX12 or h12-LOX) has been implicated in a variety of human diseases. The p...
Human platelet 12-lipoxygenase (hp-12LOX, 662 residues+iron nonheme cofactor) and its major metaboli...
It was previously shown that human platelet 12S-lipoxygenase (h12-LOX) exists as a dimer; however, t...
Human platelet 12-(S)-Lipoxygenase (12-LOX) is a fatty acid metabolizing oxygenase that plays an imp...
The research contained in this dissertation examines the kinetic, mechanistic, and structural basis ...
Mammalian lipoxygenases belong to a family of lipid-peroxidizing enzymes, which have been implicated...
12/15-Lipoxygenases (12/15-LOX) have been implicated in inflammatory and hyperproliferative diseases...
Background: Mammalian lipoxygenases (LOXs) are categorised with respect to their positional specific...
AbstractMammalian lipoxygenases play a role in normal cell development and differentiation but they ...
Research has shown that chronic inflammation is associated with many diseases such as cardiovascular...
International audiencePlant lipoxygenases (LOXs) are a class of widespread dioxygenases catalyzing t...
a b s t r a c t Mammalian lipoxygenases play a role in normal cell development and differentiation b...
SummaryLipoxygenases are critical enzymes in the biosynthesis of families of bioactive lipids includ...
ABSTRACT: 15-Lipoxygenases are lipid-peroxidizing enzymes which have been implicated in the pathogen...
Lipoxygenases (LOX) play critical roles in mammalian biology in the generation of potent lipid media...
Human platelet ALOX12 (hALOX12 or h12-LOX) has been implicated in a variety of human diseases. The p...
Human platelet 12-lipoxygenase (hp-12LOX, 662 residues+iron nonheme cofactor) and its major metaboli...
It was previously shown that human platelet 12S-lipoxygenase (h12-LOX) exists as a dimer; however, t...
Human platelet 12-(S)-Lipoxygenase (12-LOX) is a fatty acid metabolizing oxygenase that plays an imp...
The research contained in this dissertation examines the kinetic, mechanistic, and structural basis ...
Mammalian lipoxygenases belong to a family of lipid-peroxidizing enzymes, which have been implicated...
12/15-Lipoxygenases (12/15-LOX) have been implicated in inflammatory and hyperproliferative diseases...
Background: Mammalian lipoxygenases (LOXs) are categorised with respect to their positional specific...
AbstractMammalian lipoxygenases play a role in normal cell development and differentiation but they ...
Research has shown that chronic inflammation is associated with many diseases such as cardiovascular...
International audiencePlant lipoxygenases (LOXs) are a class of widespread dioxygenases catalyzing t...
a b s t r a c t Mammalian lipoxygenases play a role in normal cell development and differentiation b...
SummaryLipoxygenases are critical enzymes in the biosynthesis of families of bioactive lipids includ...
ABSTRACT: 15-Lipoxygenases are lipid-peroxidizing enzymes which have been implicated in the pathogen...
Lipoxygenases (LOX) play critical roles in mammalian biology in the generation of potent lipid media...