Integrated disperser freezing purification with extraction using fatty acid-based solidification of floating organic-droplet (IDFP-EFA-SFO) for triclosan and methyltriclosan determination in seawater, sediment and seafood.

A microextraction method for the determination of triclosan and methyltriclosan in marine environmental samples was developed. The disperser was first serves as a preliminary extractant for analytes, then as a frozen solvent to remove impurities at -20 °C, and finally as a disperser agent in the microextraction procedure. With the extractants solidified and float on the surface of the aqueous phase at low temperature, a separation was achieved to avoided use of specialized laboratory instruments. The method was optimized using Plackett-Burman design and central composite design as follows: 146 μL octanoic acid as extractant, 793 μL acetoneas disperser, 3.0 min centrifugation and 1.1 min vortex time. The limits of detection were 0.022-0.060 μg L-1 or μg kg-1 and recoveries were 83.3-103.5% for TCS and MTCS in seawater, sediments and seafood. The method has excellent prospects for sample pre-treatment and trace-level analysis of triclosan and methyltriclosan in marine environmental samples