Eukaryotic cells rely on long-lived microtubules for intracellular transport and as compression-bearing elements. We considered that long-lived microtubules are acetylated inside their lumen and that microtubule acetylation may modify microtubule mechanics. Here, we found that tubulin acetylation is required for the mechanical stabilization of long-lived microtubules in cells. Depletion of the tubulin acetyltransferase TAT1 led to a significant increase in the frequency of microtubule breakage. Nocodazole-resistant microtubules lost upon removal of acetylation were largely restored by either pharmacological or physical removal of compressive forces. In in vitro reconstitution experiments, acetylation was sufficient to protect microtubules f...
Microtubules are cytoskeleton components with unique mechanical and dynamic properties. They are rig...
The post-translational modification of tubulin appears to be a highly controlled mechanism that regu...
International audienceThe dynamic instability of microtubules is characterized by slow growth phases...
Eukaryotic cells rely on long-lived microtubules for intracellular transport and as compression-bear...
International audienceLong-lived microtubules endow the eukaryotic cell with long-range transport ab...
International audienceAmong the different types of cytoskeletal components, microtubules arguably ac...
Microtubules contribute to diverse cellular processes through balancing dynamic, short-lived and sta...
Tubulin is subject to a post-translational acety-lation reaction that is thought to be correlated wi...
Microtubules, or cytoskeletal polymers composed of the protein tubulin, form long hollow tubes in th...
University of Minnesota M.S. thesis. March 2020. Major: Molecular, Cellular, Developmental Biology ...
Acetylation ofα-tubulin at lysine 40 (K40) is a well-conserved posttranslational modification that m...
New findings about posttranslational modifications of microtubules lead us to believe that microtubu...
SummaryAcetylation of α-tubulin Lys40 by tubulin acetyltransferase (TAT) is the only known posttrans...
Microtubule-based transport is required for plasmid translocation to the nucleus during transfection...
Microtubules (MTs) are highly conserved, cytoskeletal polymers that are involved in a large number o...
Microtubules are cytoskeleton components with unique mechanical and dynamic properties. They are rig...
The post-translational modification of tubulin appears to be a highly controlled mechanism that regu...
International audienceThe dynamic instability of microtubules is characterized by slow growth phases...
Eukaryotic cells rely on long-lived microtubules for intracellular transport and as compression-bear...
International audienceLong-lived microtubules endow the eukaryotic cell with long-range transport ab...
International audienceAmong the different types of cytoskeletal components, microtubules arguably ac...
Microtubules contribute to diverse cellular processes through balancing dynamic, short-lived and sta...
Tubulin is subject to a post-translational acety-lation reaction that is thought to be correlated wi...
Microtubules, or cytoskeletal polymers composed of the protein tubulin, form long hollow tubes in th...
University of Minnesota M.S. thesis. March 2020. Major: Molecular, Cellular, Developmental Biology ...
Acetylation ofα-tubulin at lysine 40 (K40) is a well-conserved posttranslational modification that m...
New findings about posttranslational modifications of microtubules lead us to believe that microtubu...
SummaryAcetylation of α-tubulin Lys40 by tubulin acetyltransferase (TAT) is the only known posttrans...
Microtubule-based transport is required for plasmid translocation to the nucleus during transfection...
Microtubules (MTs) are highly conserved, cytoskeletal polymers that are involved in a large number o...
Microtubules are cytoskeleton components with unique mechanical and dynamic properties. They are rig...
The post-translational modification of tubulin appears to be a highly controlled mechanism that regu...
International audienceThe dynamic instability of microtubules is characterized by slow growth phases...