Creation of myeloma model in balb/c mice: experimental study Balb/c farelerde miyeloma modelinin oluşturulması: Deneysel çalışma

© 2021 by Türkiye Klinikleri.Objective: Multiple myeloma (MM) disease is characterized by clonal proliferation of malignant plasma cells in the bone marrow. It represents approximately 1% of all cancers in the world. Individual dissolution foci or diffuse osteopenia are common in the bones. In approximately 3% of the cases, non-bone marrow (extramedullary) involvement may occur and this solid involvement outside the bone marrow is defined as plasmacytoma. Experimental animal studies are widely used to elucidate metabolic and physiological mechanisms. It is more advantageous to use experimental animal models since there are many systemic organ involvement, especially in hematological cancers. We aimed to create a standard animal model for MM which is suitable for new experiments and drug trials. Seven experimental animal groups were created apart from the control group. Different amount of MOPC315 cells (1-2-5x106/200-500 µL) applied to three different regions (subcutaneous right abdominal, intravenous to the tail vessel, intraperitoneal right abdominal), in male (n=10) and female (n=7) BALB/c mice. Insulin injection should not be used. Different waiting times were applied for tumor development (20-30 days). Material and Methods: The control group consisted of 1 male and 1 female mouse. Pathological examination was performed after hematoxylin and eosin staining of organs and suspicious tissues of mice killed by cervical dislocation. Results: According to our study, after cells were injected, pneumonia, which is one of the late symptoms of MM, developed in females, but no tumor was formed. Whereas, after the cell was injected, tumor formation was observed in males. Conclusion: According to our results, we suggest that the standard MM animal model can be created by intraperitoneally injecting of 2x106/500 µL MOPC315 cells into male BALB/c mouse. Our work has been a pioneer in the use of animal experiments in MM disease in Turkey