Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples
- Kaku Norihito
- Hashiguchi Kohji
- Akamatsu Norihiko
- Wakigawa Fumiko
- Matsuda Junichi
- Komaru Kenzo
- Nakao Takumi
- Harada Yosuke
- Hara Atsuko
- Uno Naoki
- Sakamoto Kei
- Morinaga Yoshitomo
- Kitazaki Takeshi
- Hasegawa Hiroo
- Miyazaki Taiga
- Fukuda Masaaki
- Izumikawa Koichi
- Mukae Hiroshi
- Yanagihara Katsunori
Publication date
February 2021
DOI Publisher
Springer Science and Business Media LLC Journal
European Journal of Clinical Microbiology & Infectious DiseasesAbstract
We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses
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