Mobilization of Dirofilaria immitis Microfilariae in Mice during the Treatment with Diethylcarbamazine

Dirofilaria immitisミクロフィラリア30,000隻をICRマウスの鼠径部皮下に接種し,Diethylcarbamazine(DEC)300mg/kg治療によるミクロフィラリアのマウス体内での分布の変動を観察した.DEC投与前の平均仔虫回収率は35.4%で,投与後3日目には21.1%に減少した.仔虫の体内での分布はDEC投与によりPeltよりCarcass, Visceraに移動する傾向がみられ,特に内臓では肝臓,腎臓,脾臓,肺臓,腸間膜等に仔虫が多くみられ,心臓では仔虫の減少が観察された.The provocative activity of diethylcarbamazine (DEC) on the skin-dwelling microfilariae was examinend by using a mouse-Dirofilaria immitis microfilariae model. The mice were inoculated subcutaneously with 30,000 D. immitis microfilariae and given DEC at dosage of 300mg/kg body weight on 3,4 and 5 days after inoculation. Ten animals treated with DEC and ten control animals were necropsied on the 6 th day after infection in order to recover microfiariae alive. DEC treatment induced the reduction in the total number of microfilariae recovered from a mouse. The remarkable reduction was recognized in the number of microfilariae from pelt (p