Leukocyte Alkaline Phosphatase in Hematological Disorders

Leukocyte alkaline phosphatase activity was studied biochemically and histochemically in various blood diseases. 1) N-APA is preferable to LAPA as a unit of biochemical measurement. 2) The low content of leukocyte AP in CGL was confirmed. The determination of leukocyte AP is useful in the diagnosis and treatment of CGL. 3) The low N-APA in CGL rises to normal as a rule after successful treatment and this normalization is consistent with the double cell population theory in leukemic processes. 4) The high content of leukocyte AP in acute leukemia, aplastic anemia, leukemoid reaction, idiopathic eosinophilia, myelosclerosis and infection may be explained by the hypothesis that it represents increased neutropoiesis. 5) A close correlation was confirmed between biochemical and histochemical results, indicating that histochemical methods are reliable and suitable for adoption as a routine test