A rapid fungal DNA extraction method suitable for PCR screening fungal mutants, infected plant tissue and spore trap samples

Publication history: Accepted - 19 November 2021; Published online - 26 November 2021.Current DNA extraction methods from filamentous fungi take from an hour to a full day. These methods require buffers and tools for grinding fungal tissues. Although commercial extraction kits can reduce the amount of time spent preparing and extracting genomic DNA from fungal samples, these can be expensive. Here we describe a quick and inexpensive sonication technique to extract genomic DNA from filamentous fungi without buffer which can be used to perform PCR in under an hour. DNA was extracted from Zymoseptoria tritici, Fusarium graminearum and Botrytis cinerea in vitro cultures, Z. tritici asexual fruiting bodies and directly from spore trap tapes.Science Foundation Ireland (SFI) 16/SPP/3296 and 15/CDA/3451