Single molecule force clamp experiments are widely used to investigate how enzymes, molecular motors, and other molecular mechanisms work. We developed a dual-trap optical tweezers instrument with real-time (200 kHz update rate) force clamp control that can exert 0–100 pN forces on trapped beads. A model for force clamp experiments in the dumbbell-geometry is presented. We observe good agreement between predicted and observed power spectra of bead position and force fluctuations. The model can be used to predict and optimize the dynamics of real-time force clamp optical tweezers instruments. The results from a proof-of-principle experiment in which lambda exonuclease converts a double-stranded DNA tether, held at constant tension, into its ...
ABSTRACT Force measurements are performed on single DNA molecules with an optical trapping interfero...
Optical tweezers has enabled application and measurement of forces in a highly quantitative way and ...
Optical tweezer is an integral tool in the research frontiers of science and engineering. In this FY...
Single molecule force clamp experiments are widely used to investigate how enzymes, molecular motors...
Optical traps are useful for studying the effects of forces on single molecules. Feedback-based forc...
Paper AbstractA double-trap optical tweezers instrument was constructed and its spatial resolution m...
ABSTRACT We constructed a next-generation optical trapping instrument to study the motility of singl...
AbstractOptical tweezers are a powerful tool for the study of single biomolecules. Many applications...
AbstractSingle molecule force spectroscopy methods, such as optical and magnetic tweezers and atomic...
AbstractWe constructed a next-generation optical trapping instrument to study the motility of single...
Optical tweezers are a means to manipulate objects with light. With the technique, microscopically s...
Optical tweezers are a means to manipulate objects with light. With the technique, microscopically s...
We have constructed an optical tweezer using two lasers (830 nm and 1064 nm) combined with micropipe...
Optical tweezers are a means to manipulate objects with light. With the technique, microscopically s...
University of Minnesota Ph.D. dissertation. April 2010. Major:Electrical Engineering. Advisor: Dr. M...
ABSTRACT Force measurements are performed on single DNA molecules with an optical trapping interfero...
Optical tweezers has enabled application and measurement of forces in a highly quantitative way and ...
Optical tweezer is an integral tool in the research frontiers of science and engineering. In this FY...
Single molecule force clamp experiments are widely used to investigate how enzymes, molecular motors...
Optical traps are useful for studying the effects of forces on single molecules. Feedback-based forc...
Paper AbstractA double-trap optical tweezers instrument was constructed and its spatial resolution m...
ABSTRACT We constructed a next-generation optical trapping instrument to study the motility of singl...
AbstractOptical tweezers are a powerful tool for the study of single biomolecules. Many applications...
AbstractSingle molecule force spectroscopy methods, such as optical and magnetic tweezers and atomic...
AbstractWe constructed a next-generation optical trapping instrument to study the motility of single...
Optical tweezers are a means to manipulate objects with light. With the technique, microscopically s...
Optical tweezers are a means to manipulate objects with light. With the technique, microscopically s...
We have constructed an optical tweezer using two lasers (830 nm and 1064 nm) combined with micropipe...
Optical tweezers are a means to manipulate objects with light. With the technique, microscopically s...
University of Minnesota Ph.D. dissertation. April 2010. Major:Electrical Engineering. Advisor: Dr. M...
ABSTRACT Force measurements are performed on single DNA molecules with an optical trapping interfero...
Optical tweezers has enabled application and measurement of forces in a highly quantitative way and ...
Optical tweezer is an integral tool in the research frontiers of science and engineering. In this FY...