Mechanical influences on endothelial cell network formation in vitro

Groothuis, Aline
Wilson, Cameron
Kasper, Grit
van Scherpenzeel, Karine
Simon, Paul
Bail, Hermann
Schuetz, Michael
Duda, Georg

Publication date

January 2010

Publisher

Georg Thieme Verlag KG

Abstract

While both the restoration of the blood supply and an appropriate local mechanical environment are critical for uneventful bone healing, their influence on each other remains unclear. Human bone fracture haematomas (<72h post-trauma) were cultivated for 3 days in fibrin matrices, with or without cyclic compression. Conditioned medium from these cultures enhanced the formation of vessel-like networks by HMEC-1 cells, and mechanical loading further elevated it, without affecting the cells’ metabolic activity. While haematomas released the angiogenesis-regulators, VEGF and TGF-β1, their concentrations were not affected by mechanical loading. However, direct cyclic stretching of the HMEC-1 cells decreased network formation. The appearance of th...

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Mechanical influences on endothelial cell network formation in vitro

Abstract

Extracted data

Mechanical influences on endothelial cell network formation in vitro

Abstract

Extracted data

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