Regeneration of Complete Plantlets from Callus Culture of Azadirachta indica A. Juss using Immature Flower Buds

Plant regeneration via callus culture was achieved using immature flower buds of mature tree of Azadirachta indica A. Juss on semi-solid MS medium enriched with 9.05µM 2,4-D + additives. The regenerated callus were amplified on lower concentration of 4.52 µM 2,4-D + additives for micro-shoot regeneration study, the best micro-shoot regenerated from callus was achieved on MS medium supplemented with 8.88 µM BAP with 3.78±0.50 numbers of shoot and 3.66±0.57 shoot length. The micro-shoots were amplified on 8.88 µM BAP+ 0.27 µM NAA with 5.22±0.53 numbers of shoot and 4.62±0.59 shoot length respectively. Micro shoot were successfully rooted on MS medium supplemented with IBA 4.92 µM with rooting percent (100%), 4.35±0.22 root number and 3.95±0.23 root length. The callus derived plantlets were hardened in vitro and ex vitro and acclimatized in poly house followed by agro-shade house. The efficiency of the protocol will be helpful for propagation of Azadirachta indica plants on a mass scale