Application of sers spectroscopy for identification of human bladder cancer

The aim of the final work is to apply surface enhanced Raman scattering methods for bladder cancer research. The tasks of this work were set as follows. 1. Prepare different colloidal nanoparticle solutions and identify the most effective for urinary bladder tissue cancer research. 2. To record the SERS spectra of the intracellular fluid collected during cytological bladder examination. 3. Make an analysis of recorded spectra and identify spectra of cancer markers. Colloidal solutions of spherical and prism silver nanoparticles produced during operation were found to have strong Raman scattering signal, and the solution prepared according to the method described by Lee and Meisel was the most potent. Analysis of SERS spectra of intercellular fluid of cancerous and healthy bladder tissues of patients showed that Amide III oscillations associated with SERS spectral bands at 1210 cm-1, with glycogen oscillations at 483 cm-1 and with lipid and protein oscillations – 1129 cm -1 are the most suitable for the identification of bladder cancer