Abstract Biological invasions are a leading cause of rapid ecological change and often present a significant financial burden. As a vibrant discipline, invasion biology has made important strides in identifying, mapping, and beginning to manage invasions, but questions remain surrounding the mechanisms by which invasive species spread and the impacts they bring about. Frequent, multiscalar ecological monitoring such as that provided through the National Ecological Observatory Network (NEON) can be an important tool for addressing some of these questions. We articulate a set of major outstanding questions in invasion biology, consider how NEON data science is positioned to contribute to addressing these questions, and provide suggestions to ...