The mechanism of DNA translocation by papillomavirus E1 and polyomavirus LTag hexameric helicases involves consecutive remodelling of subunit-subunit interactions around the hexameric ring. Our biochemical analysis of E1 helicase demonstrates that a 26-residue C-terminal segment is critical for maintaining the hexameric assembly. As this segment was not resolved in previous crystallographic analysis of E1 and LTag hexameric helicases, we determined the solution structure of the intact hexameric E1 helicase by Small Angle X-ray Scattering. We find that the C-terminal segment is flexible and occupies a cleft between adjacent subunits in the ring. Electrostatic potential calculations indicate that the negatively charged C-terminus can bridge t...
Helicases are molecular machines that utilize energy derived from ATP hydrolysis to move along nucle...
AbstractWe have determined the crystal structure of an active, hexameric fragment of the gene 4 heli...
Viruses frequently combine multiple activities into one polypeptide to conserve coding capacity. Thi...
The mechanism of DNA translocation by papillomavirus E1 and polyomavirus LTag hexameric helicases in...
The mechanism of DNA translocation by papillomavirus E1 and polyomavirus LTag hexameric helicases in...
Hexameric helicases are motor proteins that unwind double-stranded DNA (dsDNA) during DNA replicatio...
This is a freely-available open access publication. Please cite the published version which is avail...
Concerted, stochastic and sequential mechanisms of action have been proposed for different hexameric...
The E1 protein of bovine papillomavirus type-1 is the viral replication initiator protein and replic...
Hexameric helicases are processive DNA unwinding machines but how they engage with a replication for...
A prerequisite for DNA replication is the unwinding of duplex DNA catalyzed by a replicative hexamer...
E1 and T-antigen of the tumour viruses bovine papillomavirus (BPV-1) and Simian virus 40 (SV40) are ...
The E1 protein of papillomavirus is a hexameric ring helicase belonging to the AAA + family. The mec...
The papillomavirus replication protein E1 assembles on the viral origin of replication (ori) as a se...
The papillomaviruses (PV) are small dsDNA tumour viruses of significant medical importance and the p...
Helicases are molecular machines that utilize energy derived from ATP hydrolysis to move along nucle...
AbstractWe have determined the crystal structure of an active, hexameric fragment of the gene 4 heli...
Viruses frequently combine multiple activities into one polypeptide to conserve coding capacity. Thi...
The mechanism of DNA translocation by papillomavirus E1 and polyomavirus LTag hexameric helicases in...
The mechanism of DNA translocation by papillomavirus E1 and polyomavirus LTag hexameric helicases in...
Hexameric helicases are motor proteins that unwind double-stranded DNA (dsDNA) during DNA replicatio...
This is a freely-available open access publication. Please cite the published version which is avail...
Concerted, stochastic and sequential mechanisms of action have been proposed for different hexameric...
The E1 protein of bovine papillomavirus type-1 is the viral replication initiator protein and replic...
Hexameric helicases are processive DNA unwinding machines but how they engage with a replication for...
A prerequisite for DNA replication is the unwinding of duplex DNA catalyzed by a replicative hexamer...
E1 and T-antigen of the tumour viruses bovine papillomavirus (BPV-1) and Simian virus 40 (SV40) are ...
The E1 protein of papillomavirus is a hexameric ring helicase belonging to the AAA + family. The mec...
The papillomavirus replication protein E1 assembles on the viral origin of replication (ori) as a se...
The papillomaviruses (PV) are small dsDNA tumour viruses of significant medical importance and the p...
Helicases are molecular machines that utilize energy derived from ATP hydrolysis to move along nucle...
AbstractWe have determined the crystal structure of an active, hexameric fragment of the gene 4 heli...
Viruses frequently combine multiple activities into one polypeptide to conserve coding capacity. Thi...