Vasculogenic properties of adventitial Sca-1(+)CD45(+) progenitor cells in mice: a potential source of vasa vasorum in atherosclerosis
- Toledo-Flores, D.
- Williamson, A.
- Schwarz, N.
- Fernando, S.
- Dimasi, C.
- Witt, T.A.
- Nguyen, T.M.
- Puranik, A.S.
- Chue, C.D.
- Delacroix, S.
- Spoon, D.B.
- Bonder, C.S.
- Bursill, C.A.
- Di Bartolo, B.A.
- Nicholls, S.J.
- Simari, R.D.
- Psaltis, P.J.
DOIAbstract
The cellular origins of vasa vasorum are ill-defined and may involve circulating or local progenitor cells. We previously discovered that murine aortic adventitia contains Sca-1⁺CD45⁺ progenitors that produce macrophages. Here we investigated whether they are also vasculogenic. In aortas of C57BL/6 mice, Sca-1⁺CD45⁺ cells were localised to adventitia and lacked surface expression of endothelial markers (<1% for CD31, CD144, TIE-2). In contrast, they did show expression of CD31, CD144, TIE-2 and VEGFR2 in atherosclerotic ApoE(-/-) aortas. Although Sca-1⁺CD45⁺ cells from C57BL/6 aorta did not express CD31, they formed CD31⁺ colonies in endothelial differentiation media and produced interconnecting vascular-like cords in Matrigel that containe...
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