Autoantibody responses to nodal and paranodal antigens in chronic inflammatory neuropathies

Mathey, E.K.
Garg, N.
Park, S.B.
Nguyen, T.
Baker, S.
Yuki, N.
Yiannikas, C.
Lin, C.S.
Spies, J.M.
Ghaoui, R.
Barnett, M.H.
Vucic, S.
Pollard, J.D.
Kiernan, M.C.

Open link

Publication date

January 2017

DOI

10.1016/j.jneuroim.2017.05.002

Publisher

Elsevier BV

Journal

Journal of Neuroimmunology

Abstract

Autoantibodies to nodal/paranodal proteins have been reported in patients with chronic inflammatory demyelinating polyneuropathy (CIDP) and multifocal motor neuropathy (MMN). To determine the frequency of anti-paranodal antibodies in our cohort of CIDP patients and to validate the presence anti-nodal antibodies in MMN, sera were screened for IgG against human neurofascin 155, contactin-1, neurofascin 186 and gliomedin using ELISA. In CIDP patients, 7% were anti-NF155 IgG4 positive and 7% were anti-CNTN1 IgG4 positive. Positive results were confirmed using cell based assays and indirect immunofluorescence on teased nerve fibres. We did not detect IgG autoantibodies against these nodal/paranodal antigens in MMN patients.E.K. Mathey, N. Garg, ...