ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF ABELMOSCHUS MANIHOT L. MEDIK LEAF FRACTION AGAINST CCL4-INDUCED LIVER DAMAGE IN RATS

Objective: The objective of this research was to determine the antioxidant and hepatoprotective activity of Abelmoschus manihot L. Medik (AMLM) leaves in carbon tetrachloride-induced liver damage in rats (Rattus norvegicus). Methods: Samples of dried leaf were macerated using 96% ethanol solvent to obtain a viscous extract. Fractionation was then performed using ethyl acetate and n-butanol solvent. The antioxidant activity test of each fraction was done by 1,1-diphenyl-2-picrylhydrazyl assay. For the hepatoprotective activity test, the fractions were suspended in Tween 20 0.4% solution with concentrations of 10 mg/mL and 20 mg/mL. Rats were divided into 6 treatment groups consisting of five rats/group. Group I (positive control) was given an oral suspension of Curcuma® tablet, Group II (negative control) was given Tween 20 0.4% solution, and Groups III and IV (ethyl acetate fraction) and V and VI (n-butanol fraction) were given fraction suspension. On the 1st–7th days, all groups were given the solution treatment, and on the 8th day, all groups were injected with CCl4 intraperitoneally and the treatment was continued until the 11th day. On the 12th day, the blood of rats was taken and then measured the serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT). Results: The results of the antioxidant test showed that ethyl acetate and n-butanol fraction, respectively, had IC50 of 89.99 and 114.56 ppm.Measurement of SGOT showed the result for Groups I–VI, respectively, of 160±63.62, 260.53±18.98, 154.16±52.78, 177.43±13.70, 120.07±34.80, and 105.23±40.49 IU/L. Measurement of SGPT showed the result for Groups I–VI, respectively, of 101.87±29.24, 108.1±9.04, 57.73±49.05, 106.07±26.45, 66.9±20.05, and 146.63±84.89 UI/L. Conclusion: The results of this research indicated that the ethyl acetate and n-butanol fraction of AMLM leaves have the antioxidant andhepatoprotective activity