THE EFFECT OF CURCUMA XANTHORRHIZA - ETHANOL EXTRACT TO BIOFILM FORMATION OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)

Objective: This in vitro study aimed to analyze the mass ratio of single- and dual-species Streptococcus mutans and Aggregatibacteractinomycetemcomitans biofilm after exposure to Curcuma xanthorrhiza ethanol extract (Xan).Methods: A bacterial suspension in brain heart infusion medium, enriched with 0.2% sucrose, was exposed to the Xan, incubated for 18 hrs, andanalyzed using a crystal violet assay.Results: This research concluded that the minimum inhibitory concentration of ethanol-temulawak extract against S. mutans was 5%, while theminimum bactericidal concentration was 15%.Conclusions: Xan prevented biofilm formation of single-species S. mutans and dual-species S. mutans and A. actinomycetemcomitans more effectivelythan it did single-species A. actinomycetemcomitans