MODIFIED QUANTIFICATION THROUGH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS FOR CANAGLIFLOZIN AND METFORMIN HYDROCHLORIDE IN BULK AND TABLETS USING ECOFRIENDLY GREEN SOLVENTS

Objective: An accurate, precise, specific modified High-Performance Liquid Chromatography method was developed for the simultaneous quantification of canagliflozin and metformin in bulk and dosage forms. Methods: A C18 column (250 x 4.6 mm; 5 µm Phenomenex) was used with mobile phase containing 0.05% v/v triethylamine (pH 6.5): acetonitrile (45:55% v/v) at 20 °C and isocratic pump was used for elution. The flow rate was maintained at 1.2 ml/min and eluents were monitored at 215 nm which is evaluated by sharp peak. Results: The retention times of canagliflozin and metformin were 3.4 min and 12.7 min respectively and showed a good linearity in the concentration range of 40-200 µg/ml of Canagliflozin have found a correlation coefficient of 0.999 and 10-50 µg/ml of Metformin with a correlation coefficient of 0.998. The average percent recoveries were found to be 98.60% and 98.90% respectively for Canagliflozin and Metformin. The developed method follows all the validation parameters like accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability. Conclusion: The proposed method was found to provide faster retention time with sharp resolution with linearity at a lowest concentration as compared to previous methods and this method is validated as per International Conference on Harmonization guidelines and successfully applied to the simultaneous estimation of Canagliflozin and metformin in bulk and dosage forms. There was no such novel method for simultaneous estimation of Canagliflozin and metformin. Hence the developed method is suitable for industrial analysis of Canagliflozin and metformin with eco-friendly,green and less expensive solvent