DISEASE PREVENTION FUNCTIONAL FOODS FROM THE HIGH POTENTIAL MMP-2 INHIBITION ACTIVITY OF PINEAPPLE JUICE GRANULE

Objective: This study aimed to compare total phenolic acid and protein contents as well as free radical scavenging, enzyme activities of fresh and fermented pineapple juice. Methods: The dried pineapple juice, prepared by freeze-drying and granulation method, with high bioactive compounds (total phenolic acid and protein contents) and biological activities (scavenging and enzyme activities) from fresh and fermented pineapple juice was developed and evaluated. The selected granules were evaluated for their stability at the 3 different temperatures for 3 mo. The pineapple juice granules were tested for the non-cytotoxic concentration by the SRB assay and Matrix metalloproteinase 2 (MMP-2) inhibition activity on human skin fibroblast. Results: The pineapple juice granules, the single-coated granules of pineapple juice showed a higher percentage of yield but lower biological contents and activities than that triple-coated granules. The triple-coated granules of pineapple juice exhibited the highest total protein content (1.53±0.10 mg Bovine serum albumin equivalent (BSAE)) with scavenging activity (51.15±5.94 %), and enzyme activity (4156.18±74.78 Casein digestion unit (CDU)/mg). For gelatinolytic activity on MMP-2, the freeze-dried powder and triple-coated granules of pineapple juice indicated the highest MMP-2 inhibition activity of 50.74% and 48.48%, respectively. Conclusion: The triple-coated granules of pineapple juice could be served as a disease prevention functional food due to its easy operation, high total phenolic acid, and protein contents with high potential MMP-2 inhibition activity