COMPARATIVE COMPOSITIONS AND ANTIBACTERIAL ACTIVITY OF THE ESSENTIAL OILS OF ANTHEMIS NOBILIS L. AND ANTHEMIS MIXTA L. (ASTERACEAE)

Objective: To evaluate the chemical composition of essential oils from aerial parts of Anthemis nobilis (An) and Anthemis mixta L (Am). and investigate their antibacterial property.Methods: The essential oils were isolated by hydro distillation and analyzed by GC and GC-MS. The disc diffusion and Agar dilution methods were used to screen the antibacterial activity against referenced and/or clinically isolated (HS) strains of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica, Klebsiella pneumonia and Shighuelle sonnei.Results: The main constituents of An essential oil were 3-methyl-2-buten-1-yl cyclopropane carboxylate (29.3%), vinyl-2,2-dimethylbutanoate (24.2%), glycidyl methacrylate (9.9%), 2-methylbutyl-2-mehylbutyrate (9.1%), isobutyl isobutyrate (7.8%) and 3,3,4-trimethylhexane (6.2%), while Am essential oil was mainly characterized by α-thujone (51.8%), β-thujone (14.6%), borneol (7.3%) and 3-hexen-1-ol (4.9%). An and Am essential oils exhibited the best antibacterial activity against the following strains of Escherichia coli ATCC 25922 (25±1.20, 32±1.00 mm), Pseudomonas aeruginosa ATCC 27853 (23±0.87, 32±0.76 mm) and Staphylococcus aureus ATCC 43300 (21±1.44, 25±1.24 mm inhibition zone diameters, respectively). Minimum inhibitory concentration (MICs) values of these oils were ranged from 32-128µg/ml.Conclusion: From this study, it can be concluded that the chemical composition of the essential oils was related to their antibacterial activity.Keywords: Anthemis nobilis L., Anthemis mixta L., Chemical composition, Antibacterial activit