VALIDATION OF ACCUMULATION OF CAMPTOTHECIN CONTENT, AN ANTI-CANCER ALKALOID IN NOTHAPODYTES NIMMONIANA GRAHAM. IN PHENOTYPIC VARIANTS: METHOD FOR IDENTIFYING HIGH-YIELDING SOURCES OF ALKALOID

Objective: The study was aimed to find the CPT accumulation, in particular organ, via variable extraction method and in vitro propagation method both qualitatively and quantitatively.Methods: In the present study, the seven phenotypic variants of N. nimmoniana such as dry leaf, fresh barks of plant, induced callus, somatic embryo and suspension culture (In exogenous medium and in pallet) in 100 mg/ml were analyzed for CPT content by TLC (Thin layer chromatography) and HPLC (High performance liquid chromatography).Results: TLC and HPLC analysis confirm the presence of CPT in dry leaf, fresh barks of the plant, induced callus and somatic embryo. Out of which bark extracts possessed a maximum amount of CPT, i.e. 377.77 µg/g, which supposed to be the first choice of CPT source.Conclusion: Results conclude that the CPT is present in all samples used except exogenous suspension culture, out of which soxhlet bark extract possesses the maximum amount of CPT