EVALUATION OF ANTICHOLINERGIC, ANTIDIABETIC AND ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF OCHNA OBTUSATA DC USING IN VITRO ASSAYS

Objective: To study the antidiabetic, anticholinergic and antioxidant activity of leaf extracts of Ochna obtusata DC. using in vitro methods.Methods: Alcoholic extract and a decoction of Ochna obtusata DC. leaf was screened for antidiabetic, anticholinergic and antioxidant activity by using in vitro methods. Total phenolic and total antioxidant capacity of Ochna obtusata DC. leaf extracts were quantified as gallic acid equivalents, using the spectrophotometric method.Results: The ethanol extract and water decoction showed strong inhibition to 1,1-diphenyl-2-picrylhydrazyl radical (IC50 0.3±0.01 µg/ml, 90±5.1 µg/ml), Nitric Oxide (IC50 of 43±1 µg/ml, 82±1 µg/ml) hydrogen peroxide(IC50 of 45±2.1 µg/ml, 92±1.3 µg/ml), hydroxyl radical (IC50 of 22±2.3 µg/ml, above 200), lipid peroxidation (IC50 of 4.37±0.5 µg/ml, 1.68±0.3 µg/ml)equivalents of gallic acid (EGA), whereas the Acetylcholinesterase (IC50 of 0.5±0.2, 40±2.12) and α-Glucosidase IC50 of 3.5±.04 µg/ml, 45±3.03 µg/ml against the standard drug (acarbose equivalents) respectively. The present investigation revealed that the alcoholic extract of Ochna obtusata DC (except lipid peroxide) is effective than water decoction.Conclusion: The present observations suggest that Ochna. obtusata DC leaves may be considered as a new source for anticholinergic, antidiabetic and antioxidant constituents for therapeutic use.Keywords: Ochna obtusata DC, Acetylcholinesterase, α-Glucosidase, Antioxidan