ANTI-OBESITY EFFECT OF RESVERATROL IN RATS ON HIGH FAT DIET THROUGH REGULATION OF GENE EXPRESSION OF VISCERAL WHITE ADIPOSE TISSUE

Objective: Obesity is a chronic disease associated with serious health complications including inflammation, insulin resistance, metabolic syndrome, cardiovascular complications and several types of cancers. So this study was carried out to look for effective intervention, especially with its rising prevalence in adults and children and lack of well-tolerated therapy.Methods: Rats received high-fat diet for four months to induce obesity. Animals which had ≥30 % increase in body weight were selected in this study. Obese rats were treated for 8 w with calorie restriction (25 % food restriction of commercial chow) and/or resveratrol (30 mg/kg/day orally).Results: Obese rats demonstrated a significant elevation in the body and visceral white adipose tissue (WAT) weight, serum total cholesterol (TC), triacylglycerol (TAG) and low-density lipoprotein cholesterol (LDL-c). Visceral WAT gene expression of fatty acid synthase (FAS) significantly increased. A remarkable decrease in gene expression of peroxisome proliferator-activated receptor gamma (PPARɣ), lipoprotein lipase (LPL) and silent information regulation 2 homolog 1 (Sirt1) was recorded. The combination significantly decreased body and visceral WAT weights, TC, TAG, LDL-C and FAS mRNA expression and increased adiponectin level, the mRNA expression of PPARɣ and Sirt1as compared to individual treatments. While all treatment strategies showed a similar increase in LPL mRNA expression.Conclusion: Calorie restriction supported with resveratrol has beneficial effects in terms of reducing body weight, and appears to reverse high fat diet-induced dysregulation of expression of the genes responsible for healthy visceral WAT. Keywords: Obesity, Visceral white adipose tissue, Calorie restriction, Resveratrol