ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF RIBES GLACIALE WALL EXTRACTS

Objective: Ribes glaciale Wall. is used in folk medicine by the locals of North West Himalayas, Uttarakhand, India, but its pharmacological potentials are not well studied. This work evaluates antioxidant, anti-inflammatory and analgesic activity of the extract of Ribes glaciale Wall.Methods: Antioxidant activity was assessed by studying free radical scavenging ability, total antioxidant capacity, ferrous ion chelation and reducing power of the extracts. The anti-inflammatory and analgesic activity was assessed by carrageenan induced rat paw edema and acetic acid induced writhing model respectively.Results: The methanolic and ethyl acetate extracts of Ribes glaciale Wall. Consists considerable amount of total phenolic and flavonoid contents. The ethyl acetate extract was potent in scavenging DPPH free radical (IC50 55.7 μg/ml) while methanolic extract demonstrated higher ability to scavenge ABTS free radical (TEAC 11802.2). Methanolic extract exhibited higher ferrous ion chelation activity (61% at 400 µg/ml). The ethyl acetate extract demonstrated superior reducing power and total antioxidant capacity. The methanolic extract was evaluated for anti-inflammatory and analgesic activity. Administration of methanolic extract of Ribes glaciale exhibited dose dependent inhibition of paw edema induced by carrageenan and also showed significant reduction in number of acetic acid induced abdominal writhing.Conclusion: The study revealed antioxidant potential of methanolic and ethyl acetate extract of Ribes glaciale Wall. The methanolic extract exhibited significant anti-inflammatory and analgesic activity and indicate the need for its further phytochemical evaluation.Â