STUDIES ON β-CYCLODEXTRIN PRODUCTION FROM CGTase PRODUCING BACTERIA AND ITS EFFECT ON DRUG SOLUBILITY

Objective: To produce cyclodextrin (CD) from Cyclodextrin glycosyltransferase (CGTase) producing bacteria isolated from various samples and to study its effect on drug solubility. Methods: CGTase producing bacteria were isolated from various samples viz. Soil, rotten potatoes, and stale corn flour dough using Horikoshi - phenolphthalein agar. All the isolates were screened for CGTase activity. Isolate showing highest CGTase activity was characterized morphologically and biochemically. CD was produced using CGTase which was further used for drug solubility studies. Results: A total of fifty samples were studied of which 20 bacterial isolates showed the presence of halo around them. Upon further screening, the culture supernatant of 12 isolates showed cyclization activity, of which CD 18 strain produced highest amount of CGTase. Morphological and biochemical characterization revealed that the isolate belonged to Bacillus sp. The isolate showed maximum growth, reducing sugars 0.724 mg/ml and maximum enzymatic activity for dextrinisation as well as cyclisation viz. 9.45 U/ml and 7.41 U/ml respectively after 48 hrs of incubation. Thus, Bacillus sp. CD 18 was used to produce CGTase, which was further used for the production of CD. CD increased the solubility of acclofenac and paracetamol. Conclusion: Bacteria are regarded as important sources of CGTases. New strains might suit better for industrial production of CGTase after optimizing the various cultures and process parameters